Department of Surgical Oncology, The Affiliated Hospital of Qinghai University, Xining, China.
Graduate School of Qinghai University, Xining, China.
Pol J Microbiol. 2022 May 31;71(2):217-226. doi: 10.33073/pjm-2022-019.
Breast cancer (BC) and benign breast lesions (BBLs) are common diseases in women worldwide. The gut microbiota plays a vital role in regulating breast diseases' formation, progression, and therapy response. Hence, we explored the structure and function of gut microflora in patients with BC and BBLs. A cohort of 66 subjects was enrolled in the study. Twenty-six subjects had BC, 20 subjects had BBLs, and 20 matched healthy controls. High throughput 16S ribosomal RNA (16S rRNA) gene sequencing technology was used to determine the microbial community structure. Compared with healthy individuals, BC patients had significantly lower alpha diversity indices (Sobs index, = 0.019; Chao1 index, = 0.033). Sobs and Chao1 indices were also lower in patients with BBLs than healthy individuals, without statistical significance ( = 0.279, = 0.314, respectively). Both unweighted and weighted UniFrac analysis showed that beta diversity differed significantly among the three groups ( = 3.376e-14, < 0.001, respectively). Compared with healthy individuals, the levels of and were higher in BC patients ( = 0.004, = 0.007, respectively), whereas and were more enriched in the benign breast lesion group ( < 0.001, = 0.011, respectively). Our study indicates that patients with BC and BBLs may undergo significant changes in intestinal microbiota. These findings can help elucidate the role of intestinal flora in BC and BBLs patients.
乳腺癌 (BC) 和良性乳腺病变 (BBL) 是全球女性常见的疾病。肠道微生物群在调节乳腺疾病的发生、发展和治疗反应方面起着至关重要的作用。因此，我们探索了 BC 和 BBL 患者肠道微生物群的结构和功能。本研究纳入了 66 名受试者。26 名受试者患有 BC，20 名受试者患有 BBL，20 名匹配的健康对照。采用高通量 16S 核糖体 RNA (16S rRNA) 基因测序技术确定微生物群落结构。与健康个体相比，BC 患者的 alpha 多样性指数 (Sobs 指数， = 0.019；Chao1 指数， = 0.033) 显著降低。BBL 患者的 Sobs 和 Chao1 指数也低于健康个体，但无统计学意义 ( = 0.279， = 0.314)。未加权和加权 UniFrac 分析均显示三组间 beta 多样性差异显著 ( = 3.376e-14， < 0.001)。与健康个体相比，BC 患者的 和 水平更高 ( = 0.004， = 0.007)，而良性乳腺病变组中 和 更为丰富 ( < 0.001， = 0.011)。本研究表明，BC 和 BBL 患者的肠道微生物群可能发生显著变化。这些发现有助于阐明肠道菌群在 BC 和 BBL 患者中的作用。
