Key Laboratory of Jiangxi Province for Research On Active Ingredients in Natural Medicines, Yichun, 33600, Jiangxi, China.
College of Chemistry and Bioengineering, Yichun University, Yichun, 336000, Jiangxi, China.
Folia Microbiol (Praha). 2022 Dec;67(6):851-860. doi: 10.1007/s12223-022-00981-6. Epub 2022 Jun 9.
Cordycepin is an essential nucleoside antibiotic with a broad spectrum of physiological functions, which is currently produced by the fermentation of Cordyceps militaris. Even though numerous efforts were made to enhance cordycepin production, the cordycepin yield is still limited. High-cordycepin-yielding strains are still a prerequisite for industrial cordycepin production in large amounts. Screening high-cordycepin-yielding strains from other sources may break new grounds for cordycepin. In this study, Cordyceps hawkesii Gray, with high homology to C. militaris, was selected as the source to screen the cordycepin manufacturing endophytic fungi. Four isolates capable of cordycepin production were successfully obtained among all isolated endophytic fungi. One of the four with better cordycepin yield was identified as Irpex lacteus CHG05, which belongs to the Phlebia species. The response surface methodology was applied to optimize the culture conditions for cordycepin fermentation. 162.05 mg/L of cordycepin with a 53.1% improvement was achieved compared to the original conditions. This study indicates that the endophytic fungi from C. hawkesii Gray could produce cordycepin and served as the first report for cordycepin by the white-rot fungus of I. lacteus. Even though the yield is low compared to C. militaris, this strain provided another choice for enhanced cordycepin in the future.
蛹虫草素是一种具有广泛生理功能的必需核苷类抗生素，目前主要通过蛹虫草发酵生产。尽管人们已经做出了许多努力来提高蛹虫草素的产量，但蛹虫草素的产量仍然有限。高产蛹虫草素的菌株仍然是大量工业化生产蛹虫草素的前提。从其他来源筛选高产蛹虫草素的菌株可能为蛹虫草素的生产开辟新的途径。在本研究中，选择与蛹虫草具有高度同源性的蝙蝠蛾被毛孢作为筛选蛹虫草素产生内生真菌的来源。从所有分离的内生真菌中成功获得了 4 株能够产生蛹虫草素的菌株。其中一株蛹虫草素产量较高的菌株被鉴定为 Phlebia 属的亮革侧耳 CHG05。响应面法被应用于优化蛹虫草素发酵的培养条件。与原始条件相比，蛹虫草素的产量提高了 53.1%，达到了 162.05mg/L。本研究表明，来自 C. hawkesii Gray 的内生真菌可以产生蛹虫草素，这是首次报道亮革侧耳产生蛹虫草素。尽管与蛹虫草相比产量较低，但该菌株为未来提高蛹虫草素产量提供了另一种选择。
