Pollard Charley-Lea, Gibb Zamira, Clulow Jennifer, Ruiz Agustin, Sheridan Alecia, Bahrami Mohammad, Swegen Aleona, Grupen Christopher G
Sydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW 2570, Australia.
Priority Research Centre for Reproductive Science, University of Newcastle, Callaghan, NSW 2308, Australia.
Animals (Basel). 2022 May 27;12(11):1383. doi: 10.3390/ani12111383.
A deficiency in NAD has previously been linked with increased occurrences of congenital abnormalities and embryonic death in humans and mice. Early embryonic death is a major factor involved in pregnancy loss in mares, and very little is known regarding the NAD requirements for optimum reproductive function in horses. The aim of this study was to determine the effect of supplementing the diet of mares with nicotinic acid (NA) on the composition of NAD metabolites in the blood and follicular fluid. Vehicle alone or NA (3 g per os) were administered to seven mares over a minimum of 3 consecutive days during the follicular phase of the oestrous cycle. Blood samples were collected immediately prior to supplemental feeding and follicular fluid aspiration. Follicular fluid was collected from the dominant follicle through transvaginal ultrasound-guided aspiration. Blood and follicular fluid samples were processed and analysed by mass spectrometry. The concentration of nicotinamide mononucleotide (NMN) in the follicular fluid of NA-fed mares was 4-fold greater than that in the corresponding plasma and 10-fold greater than that in the follicular fluid of vehicle-fed mares. The concentrations of NA, nicotinamide (NAM) and nicotinuric acid (NUR) tended to be greater in the follicular fluid of NA-supplemented mares than in the corresponding plasma. The results show that NA supplementation increased the bioavailability of NAD precursors in the follicular fluid of the dominant follicle, which is proposed to better promote the maturation of good quality oocytes, especially in older mares.
此前已发现，烟酰胺腺嘌呤二核苷酸（NAD）缺乏与人类和小鼠先天性异常及胚胎死亡发生率增加有关。早期胚胎死亡是母马妊娠丢失的一个主要因素，而关于马匹最佳生殖功能所需的NAD水平，人们了解甚少。本研究的目的是确定在母马日粮中补充烟酸（NA）对血液和卵泡液中NAD代谢物组成的影响。在发情周期的卵泡期，给7匹母马连续至少3天单独灌服赋形剂或口服3克NA。在补充饲喂和卵泡液抽吸前立即采集血样。通过经阴道超声引导抽吸从优势卵泡收集卵泡液。对血液和卵泡液样本进行处理并通过质谱分析。补充NA的母马卵泡液中烟酰胺单核苷酸（NMN）的浓度比相应血浆中的浓度高4倍，比灌服赋形剂的母马卵泡液中的浓度高10倍。补充NA的母马卵泡液中NA、烟酰胺（NAM）和烟尿酸（NUR）的浓度往往高于相应血浆中的浓度。结果表明，补充NA提高了优势卵泡卵泡液中NAD前体的生物利用度，这被认为可以更好地促进优质卵母细胞的成熟，尤其是在老龄母马中。
