Nakashima K, Takei H, Nasu Y, Andoh Y
Clin Chem. 1987 May;33(5):708-10.
We evaluated whether any monosaccharides inhibit glycolysis in erythrocytes and discovered that D-mannose does. In the presence of D-mannose, glucose can be accurately measured by either the hexokinase procedure or the glucose oxidase procedure. In comparison studies with other glucose preservatives, we found that after 2 h at room temperature glucose decreased by 21 (SD 13) mg/L in D-mannose-treated blood, 93 (SD 10) mg/L in sodium fluoride-treated blood, 28 (SD 21) mg/L in ice-cooled blood, and 144 (SD 28) mg/L in control blood (no preservative treatment). Because D-mannose acted in the early phase of glycolysis, it was a more effective preservative than sodium fluoride; moreover, its use did not preclude measurement of sodium and potassium in the blood samples. D-Mannose did not interfere with other routine chemical tests except for the assay of creatine kinase involving coupled enzymes hexokinase/glucose-6-phosphate dehydrogenase. Creatine kinase could be correctly assayed in the presence of D-mannose by using glucokinase instead of hexokinase. D-Mannose can be used with or without anticoagulant and is compatible with most types of multi-channel automated analyzers.
我们评估了是否有单糖能抑制红细胞中的糖酵解，结果发现D-甘露糖可以。在D-甘露糖存在的情况下，可以通过己糖激酶法或葡萄糖氧化酶法准确测量葡萄糖。在与其他葡萄糖防腐剂的对比研究中，我们发现，在室温下放置2小时后，D-甘露糖处理的血液中葡萄糖降低了21（标准差13）mg/L，氟化钠处理的血液中降低了93（标准差10）mg/L，冰冷却血液中降低了28（标准差21）mg/L，对照血液（未进行防腐剂处理）中降低了144（标准差28）mg/L。由于D-甘露糖作用于糖酵解的早期阶段，它是一种比氟化钠更有效的防腐剂；此外，使用它并不妨碍对血样中的钠和钾进行测量。除了涉及偶联酶己糖激酶/葡萄糖-6-磷酸脱氢酶的肌酸激酶测定外，D-甘露糖不干扰其他常规化学检测。通过使用葡萄糖激酶代替己糖激酶，可以在D-甘露糖存在的情况下正确测定肌酸激酶。D-甘露糖可与抗凝剂一起使用或不使用抗凝剂，并且与大多数类型的多通道自动分析仪兼容。
