Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, 50 Ngamwongwan Road, Chatuchak, Bangkok 10900, Thailand.
Nutrients. 2022 May 28;14(11):2275. doi: 10.3390/nu14112275.
The present study investigated the gut health, anti-diabetic, and anti-inflammatory activities of mung bean seed coat extract (MSE). MSE was obtained by pressurized liquid extraction (PLE) using 50% ethanol as the extracting solvent. After 24 h of in vitro human fecal fermentation, MSE exhibited higher productions of total short-chain fatty acids (SCFA) than those of the control group (CON) and other polyphenol-rich substrates, including gallic acid (GA) and vitexin (VIT) (p > 0.05), but still lower than the fructo-oligosaccharide (FOS). In 16S-rRNA next-generation sequencing, MSE regulated the composition of gut microbiota by stimulating the growth of the beneficial bacteria Enterococcus, Ruminococcus, Blautia, and Bacteroides and decreasing the growth of the potential pathogenic bacteria Escherichia-Shigella. Similarly, qPCR showed increased numbers of Bifidobacterium, Lactobacillus, Faecalibacterium prausnitzii, and Prevotella, compared with those of CON (p < 0.05). MSE also reduced reactive oxygen species and increased glucose uptake in insulin-resistant HepG2 cells dose-dependently. The anti-inflammatory activity of MSE was observed in LPS-stimulated THP-1 monocytes with the reduction of TNFα, IL-1β, IL-6, and IL-8 genes. The data demonstrated the potential applications of MSE as a dietary supplement with gut health benefits and its ability to mitigate diabetes and inflammatory-related diseases.
本研究考察了绿豆种皮提取物（MSE）对肠道健康、抗糖尿病和抗炎活性的影响。MSE 通过加压液体提取（PLE）用 50%乙醇作为提取溶剂获得。在体外人体粪便发酵 24 小时后，MSE 表现出比对照组（CON）和其他富含多酚的基质（包括没食子酸（GA）和牡荆素（VIT））更高的总短链脂肪酸（SCFA）产量（p>0.05），但仍低于果寡糖（FOS）。在 16S-rRNA 下一代测序中，MSE 通过刺激有益细菌肠球菌、瘤胃球菌、布劳特氏菌和拟杆菌的生长并降低潜在致病菌大肠杆菌-志贺氏菌的生长来调节肠道微生物群落的组成。同样，qPCR 显示与 CON 相比，双歧杆菌、乳杆菌、普拉梭菌和普雷沃特氏菌的数量增加（p<0.05）。MSE 还可剂量依赖性地减少活性氧并增加胰岛素抵抗 HepG2 细胞的葡萄糖摄取。MSE 在 LPS 刺激的 THP-1 单核细胞中表现出抗炎活性，降低了 TNFα、IL-1β、IL-6 和 IL-8 基因的表达。这些数据表明 MSE 作为一种具有肠道健康益处的膳食补充剂具有潜在的应用前景，并且能够减轻糖尿病和炎症相关疾病。
