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利用 SPME-GC-MS 对农林复合系统下花朵的花香化合物进行比较分析。

Comparative Analysis of the Floral Fragrance Compounds of Flowers under the - Agroforestry System Using SPME-GC-MS.

机构信息

Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650224, China.

Department of Horticulture, College of Landscape Architecture and Horticulture, Southwest Forestry University, Kunming 650224, China.

出版信息

Molecules. 2022 Jun 1;27(11):3565. doi: 10.3390/molecules27113565.

Abstract

is a medicinal plant in China, the flowers of which have high medicinal value. To study the differences in the floral fragrance compounds of flowers (bionic wild cultivation) from the forests of Yunnan Province, the floral fragrance compounds from four varieties of flowers (four-forked seven leaves, three-forked seven leaves, four-forked five-seven leaves, and three-forked five-six leaves) were compared and analyzed via headspace solid phase microextraction combined with gas chromatography-mass spectrometry methods. A total of 53 floral fragrance compounds from the flowers were divided into eight categories, mainly consisting of terpenes, alkynes, aromatic hydrocarbons, and alcohols. Moreover, high contents of 3-carene, germacrene D, (-)-α-gurjunene, valencene, (+)--gurjunene, menogene, and aromandendrene were identified from the flowers of different varieties. Interestingly, floral fragrance compounds such as 3-carene, valencene, aromandendrene, menogene, and (+)--gurjunene were first reported in the flowers of . Cluster analysis showed that with four-forked and three-forked leaves clustered into two subgroups, respectively. In addition, principal component analysis showed that (+)--gurjunene, (+)-calarene, copaene, 1,8,12-bisabolatriene, -elemene, (-)-aristolene, caryophyllene, 3-carenes, and 2,6-dimethyl-1,3,6-heptatriene can be used to distinguish the floral fragrance components of four flower species. This study provides a theoretical basis for elucidating the floral fragrance compounds emitted from the flowers of different varieties in an agroforestry system.

摘要

是中国的一种药用植物,其花朵具有很高的药用价值。为了研究云南省森林中仿生栽培的与野生的花朵在花香化合物上的差异,采用顶空固相微萃取结合气相色谱-质谱联用的方法,对四个品种(四叉七叶、三叉七叶、四叉五-七叶、三叉五-六叶)的花香化合物进行了比较分析。共从花朵中分离出 53 种花香化合物,分为萜烯类、炔烃类、芳烃类和醇类等 8 类。此外,从不同品种的花朵中鉴定出了大量的β-蒎烯、大根香叶二烯 D、(-)-α-古巴烯、石竹烯、β-金合欢烯、檀香醇和莪术烯。有趣的是,3-蒈烯、石竹烯、莪术烯、檀香醇和β-金合欢烯等花香化合物在 中的花朵中首次被报道。聚类分析表明,具有四叉和三叉叶的分别聚为两个亚组。此外,主成分分析表明,(+)-古巴烯、(+) - 蒈烯、毕澄茄烯、1,8,12-倍半蒈二烯、β-榄香烯、(-)-莪术二烯、石竹烯、3-蒈烯和 2,6-二甲基-1,3,6-庚三烯可用于区分四个品种的花香成分。本研究为阐明农林复合系统中不同品种花朵散发的花香化合物提供了理论依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e8ee/9182305/f415fb416256/molecules-27-03565-g0A1.jpg

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