Liang Xuelei, Li Jiaying, Yan Yan, Xu Yongsheng, Wang Xiujuan, Wu Haixuan, Liu Yi, Li Linfeng, Zhuo Fenglin
Department of Dermatology, Beijing Friendship Hospital, Capital Medical University, Beijing, China.
Department of Dermatology, Xuanwu Hospital, Capital Medical University, Beijing, China.
Front Med (Lausanne). 2022 May 24;9:837332. doi: 10.3389/fmed.2022.837332. eCollection 2022.
Fighting skin aging signs is one of the major challenges of the 21st century, recently, mesenchymal stem cells (MSCs) and microneedling (MN) have been applied for anti-aging. This study aims to evaluate the efficacy of the combination of MN and human umbilical cord-derived mesenchymal stem cells conditioned media (hUC-MSCs-CM) in skin brightness and rejuvenation.
Thirty volunteers with facial skin aging were recruited for the randomized, controlled split-face study. The left and right sides of the face were randomly applied with saline MN or hUC-MSCs-CM MN. Five sessions were performed for each volunteer at 2-week intervals. Two dermatologists evaluated the clinical improvement, in terms of skin brightness and texture. A satisfaction score based on a self-evaluation questionnaire was recorded at 2 weeks after the last treatment. The objective evaluation was recorded before the first treatment, and at 2 weeks after the last treatment.
Twenty-eight volunteers with a mean (SD) age of 41 (6.54) years old completed the trial. The investigator's assessment for skin brightness and texture, and the self-satisfaction score revealed statistically better effects in hUC-MSCs-CM -plus-MN group than in MN alone (MN saline) group. No severe side effects were reported during the whole study period. Compared to MN alone group, the objective assessment revealed significant improvements in skin brightness (reduced melanin index, ultraviolet spots, and brown spots) and skin texture (reduced wrinkles and pores, and increased skin elasticity) in hUC-MSCs-CM-plus-MN group, while there were no obvious differences in skin hydration, epidermal water loss, and the erythema index.
The combination of hUC-MSCs-CM and MN exhibite anti-aging efficacy, and this could be used for facial rejuvenation in the future.
对抗皮肤衰老迹象是21世纪的主要挑战之一。最近，间充质干细胞（MSCs）和微针（MN）已被应用于抗老化。本研究旨在评估微针与人类脐带间充质干细胞条件培养基（hUC-MSCs-CM）联合使用对皮肤亮度和年轻化的效果。
招募30名面部皮肤老化的志愿者进行随机对照的面部双侧研究。面部的左侧和右侧随机分别涂抹生理盐水+微针或hUC-MSCs-CM+微针。每位志愿者每隔2周进行5次治疗。两名皮肤科医生从皮肤亮度和质地方面评估临床改善情况。在最后一次治疗后2周记录基于自我评价问卷的满意度评分。客观评估在第一次治疗前以及最后一次治疗后2周进行记录。
28名平均（标准差）年龄为41（6.54）岁的志愿者完成了试验。研究者对皮肤亮度和质地的评估以及自我满意度评分显示，hUC-MSCs-CM加微针组的效果在统计学上优于单纯微针（微针+生理盐水）组。在整个研究期间未报告严重副作用。与单纯微针组相比，客观评估显示hUC-MSCs-CM加微针组在皮肤亮度（黑色素指数降低、紫外线斑和褐斑减少）和皮肤质地（皱纹和毛孔减少、皮肤弹性增加）方面有显著改善，而在皮肤水合作用、表皮水分流失和红斑指数方面没有明显差异。
hUC-MSCs-CM与微针联合使用具有抗老化功效，未来可用于面部年轻化。
