Arafa Amany A, Hedia Riham H, Dorgham Sohad M, Ibrahim Eman S, Bakry Magdy A, Abdalhamed Abeer M, Abuelnaga Azza S M
Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt.
Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Egypt.
Vet World. 2022 Apr;15(4):827-833. doi: 10.14202/vetworld.2022.827-833. Epub 2022 Apr 6.
The World Health Organization considers multidrug-resistant (MDR) a major global threat. Horses harbor commensal isolates of this bacterial species and potentially serve as reservoirs for human MDR bacteria. This study investigated antimicrobial resistance in horses caused by extended-spectrum β-lactamase (ESBL)-producing .
One hundred fifty-nine nasal swab samples were collected from horses with respiratory distress not treated with cefotaxime and erythromycin. Biochemical and serological identification was performed on all samples. Polymerase chain reaction (PCR) was used to detect , mucoviscosity-associated gene gA), uridine diphosphate galacturonate 4-epimerase gene e), and iron uptake system gene u), , , and genes. Sequence analysis and phylogenetic relatedness of randomly selected isolates carrying the gene were performed.
Ten isolates of spp. were obtained from 159 samples, with an incidence of 6.28% (10 of 159). Based on biochemical and serological identification, was detected in 4.4% (7 of 159) of the samples. Using PCR, all tested isolates (n=7) carried the gene. By contrast, no isolates carried A, , and genes. The gene was detected in all test isolates. Moreover, all isolates did not harbor the or gene. Sequence analysis and phylogenetic relatedness reported that the maximum likelihood unrooted tree generated indicated the clustering of the test isolate with the other Gram-negative isolate . Finally, the sequence distance of the gene of the test isolate (generated by Lasergene) showed an identity range of 98.4-100% with the gene of the different test isolates.
The misuse of antimicrobials and insufficient veterinary services might help generate a population of ESBL-producing in equines and humans, representing a public health risk.
世界卫生组织认为多重耐药（MDR）是一个重大的全球威胁。马携带这种细菌物种的共生分离株，有可能成为人类MDR细菌的储存宿主。本研究调查了由产超广谱β-内酰胺酶（ESBL）的[细菌名称未给出]引起的马的抗菌药物耐药性。
从未用头孢噻肟和红霉素治疗的有呼吸窘迫症状的马身上采集了159份鼻拭子样本。对所有样本进行生化和血清学鉴定。采用聚合酶链反应（PCR）检测[细菌名称未给出]、黏液粘度相关基因gA、尿苷二磷酸半乳糖醛酸4-表异构酶基因e、铁摄取系统基因u、[其他基因名称未给出]、[其他基因名称未给出]和[其他基因名称未给出]基因。对随机选择的携带[细菌名称未给出]基因的分离株进行序列分析和系统发育相关性分析。
从159份样本中获得了10株[细菌名称未给出]属分离株，发生率为6.28%（159份中的10份）。基于生化和血清学鉴定，在4.4%（159份中的7份）的样本中检测到[细菌名称未给出]。使用PCR，所有检测的[细菌名称未给出]分离株（n = 7）都携带[细菌名称未给出]基因。相比之下，没有分离株携带A、[其他基因名称未给出]和[其他基因名称未给出]基因。在所有检测分离株中都检测到了[细菌名称未给出]基因。此外，所有分离株都未携带[其他基因名称未给出]或[其他基因名称未给出]基因。序列分析和系统发育相关性报告显示，生成的最大似然无根树表明检测分离株与其他革兰氏阴性分离株[细菌名称未给出]聚类。最后，检测分离株的[细菌名称未给出]基因的序列距离（由Lasergene生成）显示与不同检测分离株的[细菌名称未给出]基因的同一性范围为98.4 - 100%。
抗菌药物的滥用和兽医服务不足可能有助于在马和人类中产生产ESBL的[细菌名称未给出]种群，这代表着一种公共卫生风险。
