Alshehabat Musa, Hananeh Wael, Ismail Zuhair Bani, Rmilah Safwan Abu, Abeeleh Mahmoud Abu
Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan.
Department of Pathology and Public Health, Faculty of Veterinary Medicine, Jordan University of Science and Technology, Irbid 22110, Jordan.
Vet World. 2020 Dec;13(12):2793-2797. doi: 10.14202/vetworld.2020.2793-2797. Epub 2020 Dec 29.
Natural topical products have been used to enhance wound healing, especially in immunocompromised animals. The aims of this study were to evaluate and to compare the effects of moist exposed burn ointment (MEBO) and honey on the healing of full-thickness skin wounds in immunocompromised dogs.
The study was conducted using 12 adults, apparently healthy mongrel dogs. Immunosuppression was induced in six dogs by oral administration of prednisone (2 mg/kg) and azathioprine (2 mg/kg), once a day for 21 days. On each dog, a total of 9, 1.5 cm in diameter full-thickness skin circular wounds were created aseptically in the thoracolumbar area under general anesthesia using customized skin punch biopsy kit. In a random fashion, three wounds in each dog were treatment using MEBO (contains b-sitosterol, baicalin, and berberine as active ingredients in a base of beeswax and sesame oil), honey or no treatment (control), once per day for 21 days. Wounds were grossly evaluated once a day for signs of inflammation or infection. In addition, biopsy specimens and digital imaging data of each wound were obtained on days 7, 14, and 21 for histopathological evaluation of the healing process.
Wounds in immunocompromised dogs appeared to heal significantly in a slower fashion than in non-immunocompromised counterparts. Digital analysis data showed that MEBO-treated wounds expressed better epithelialization area, faster contraction, and smaller wound area percentage when compared with honey-treated wounds. Histopathological analysis showed significantly higher angiogenesis scores in MEBO-treated wounds when compared with other treatments.
Results of this study showed that MEBO resulted in significant enhancement of wound healing in both healthy and immunocompromised dogs. However, when compared to honey, the wound healing effect of MEBO was superior to that of honey.
天然外用产品已被用于促进伤口愈合，尤其是在免疫功能低下的动物中。本研究的目的是评估和比较湿润烧伤膏（MEBO）和蜂蜜对免疫功能低下犬全层皮肤伤口愈合的影响。
本研究使用了12只成年、外观健康的杂种犬。通过口服泼尼松（2mg/kg）和硫唑嘌呤（2mg/kg）对6只犬进行免疫抑制，每天一次，共21天。在每只犬的胸腰段区域，使用定制的皮肤打孔活检套件在全身麻醉下无菌创建总共9个直径为1.5cm的全层皮肤圆形伤口。以随机方式，每只犬的三个伤口分别用MEBO（含有β-谷甾醇、黄芩苷和小檗碱作为活性成分，以蜂蜡和芝麻油为基质）、蜂蜜治疗或不治疗（对照），每天一次，共21天。每天对伤口进行大体评估，观察炎症或感染迹象。此外，在第7、14和21天获取每个伤口的活检标本和数字成像数据，用于愈合过程的组织病理学评估。
免疫功能低下犬的伤口愈合速度明显比免疫功能正常的犬慢。数字分析数据显示，与蜂蜜治疗的伤口相比，MEBO治疗的伤口上皮化面积更好、收缩更快且伤口面积百分比更小。组织病理学分析显示，与其他治疗相比，MEBO治疗的伤口血管生成评分显著更高。
本研究结果表明，MEBO可显著促进健康和免疫功能低下犬的伤口愈合。然而，与蜂蜜相比，MEBO的伤口愈合效果优于蜂蜜。
