Lukiswanto Bambang Sektiari, Miranti Alya, Sudjarwo Sri Agus, Primarizky Hardany, Yuniarti Wiwik Misaco
Department of Clinical Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
J Adv Vet Anim Res. 2019 Apr 14;6(2):202-207. doi: 10.5455/javar.2019.f333. eCollection 2019 Jun.
This research was conducted to study the wound healing process of whole fruit pomegranate extract () standardized with 40% ellagic acid ointment for deep second-degree burn wound of skin in the rat ().
Powder of standardized pomegranate extract (SPE) with 40% ellagic acid was processed to become ointments. Twenty-five male rats, weighed 150-180 gm at 3 months of age, were randomly divided into five groups. After anesthetized, stainless circle plate with 1 cm of diameter in 85°C was contacted firmly toward right gluteal of rat skins for 5 sec in order to create deep second-degree burn wound. Control groups consist of (T0) cream base and (T1) 1% silver sulfadiazine. Treatment groups consist of (T2) 2.5% SPE, (T3) 5% SPE, and (T4) 10% SPE. Histopathological preparation used hematoxylin-eosin stained skin samples. Histological observations were performed using the optics microscope against collagen, the number of polymorphonuclear cell (PMN) infiltration, the degree of angiogenesis, and re-epithelization. The results were statistically compared between groups.
Microscopic observation on the wound healing process on the collagen, PMN infiltration, angiogenesis, and re-epithelization showed that topical administration of 10% SPE in burns gives the best result. This is characterized by a high density of collagen with a good arrangement, which is accompanied by a complete and mature epithelium, low number of inflammatory cells, and angiogenesis. This may be caused by the compounds in the pomegranate extract, which have the antioxidant, anti-inflammatory, and anti-bacterial effects.
This study reveals that 10% SPE accelerates the healing of deep second-degree burn wound. Thus, pomegranate standardized with 40% ellagic acid is a promising herb for the healing of burn wound of skin.
本研究旨在探讨用含40%鞣花酸的全果石榴提取物（）制成的软膏对大鼠（）皮肤深二度烧伤创面的愈合过程。
将含40%鞣花酸的标准化石榴提取物（SPE）粉末制成软膏。25只3月龄、体重150 - 180克的雄性大鼠随机分为五组。麻醉后，将直径1厘米、温度85°C的不锈钢圆盘紧贴大鼠右臀部皮肤5秒，造成深二度烧伤创面。对照组包括（T0）乳膏基质和（T1）1%磺胺嘧啶银。治疗组包括（T2）2.5% SPE、（T3）5% SPE和（T4）10% SPE。组织病理学标本采用苏木精 - 伊红染色的皮肤样本。使用光学显微镜对胶原蛋白、多形核细胞（PMN）浸润数量、血管生成程度和再上皮化进行组织学观察。对各组结果进行统计学比较。
对胶原蛋白、PMN浸润、血管生成和再上皮化的创面愈合过程进行显微镜观察表明，局部应用10% SPE治疗烧伤效果最佳。其特征为胶原蛋白密度高且排列良好，伴有完整成熟的上皮、炎症细胞数量少和血管生成。这可能是石榴提取物中的化合物具有抗氧化、抗炎和抗菌作用所致。
本研究表明10% SPE可加速深二度烧伤创面的愈合。因此，含40%鞣花酸的标准化石榴是治疗皮肤烧伤创面的一种有前景的草药。
