CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
ACS Appl Bio Mater. 2022 Jul 18;5(7):3418-3427. doi: 10.1021/acsabm.2c00361. Epub 2022 Jun 15.
As one of the typical carbon nanomaterials, graphdiyne (GDY) with unique chemical, physical, and electronic properties has a great potential in various fields. Although it is an important member of carbon nanozymes, the research on its intrinsic enzyme mimetic properties and applications is still limited. Herein, graphdiyne oxide quantum dots (GDYO QDs) have been synthesized through oxidative cleavage, which exhibit enhanced peroxidase-like activity with lower and higher than those of most carbon-based nanozymes. The catalytic mechanism is explored, showing that the enhanced catalytic performance is attributed to the good conjugated structure, large number of oxygen-containing groups, and small-sized nanosheets with few layers. As a kind of peroxidase mimetic, the GDY-based nanozyme has excellent potential in sensing HO and biological antioxidants through the colorimetric assay, with a linear range from 5 to 500 μM and detection limit of 1.5 μM for HO and a linear range from 0 to 90 μM and detection limit of 0.48 μM for l-cysteine. Our work will be beneficial to develop high-performance artificial enzymes and to understand their mechanism for better applications.
作为典型的碳纳米材料之一，具有独特的化学、物理和电子特性的二维碳材料（GDY）在各个领域都有很大的潜力。尽管它是碳纳米酶的重要成员之一，但对其内在酶模拟特性和应用的研究仍然有限。本文通过氧化裂解合成了氧化石墨炔量子点（GDYO QDs），其过氧化物酶样活性增强， 和 均低于大多数基于碳的纳米酶。本文还探讨了其催化机制，表明增强的催化性能归因于良好的共轭结构、大量的含氧基团以及具有少层的小尺寸纳米片。作为一种过氧化物酶模拟物，基于 GDY 的纳米酶通过比色测定法在检测 HO 和生物抗氧化剂方面具有优异的潜力，HO 的线性范围为 5 至 500 μM，检测限为 1.5 μM，l-半胱氨酸的线性范围为 0 至 90 μM，检测限为 0.48 μM。我们的工作将有利于开发高性能的人工酶，并深入了解其机制，以实现更好的应用。
