McSweegan E, Burr D H, Walker R I
Infect Immun. 1987 Jun;55(6):1431-5. doi: 10.1128/iai.55.6.1431-1435.1987.
An in vitro mucus assay was developed to study the role of mucus gel and secretory immunoglobulin A (sIgA) in preventing attachment of Campylobacter jejuni to INT 407 cells. An overlay of rabbit small intestinal mucus was found to impede the attachment of C. jejuni to a monolayer of INT 407 cells. Mucus from rabbits previously colonized with C. jejuni was found to completely inhibit bacterial adherence to the underlying cells. Anti-Campylobacter sIgA was readily detected in mucus samples from previously exposed rabbits and was responsible for eliminating bacterial adherence to the INT 407 cells. This was shown by loss of inhibition after mucus absorption with Campylobacter cells. sIgA-containing mucus caused aggregation of the C. jejuni cells within the mucus layer of the assay system. Nonimmune mucus and sIgA alone were unable to cause bacterial aggregation, suggesting a cooperative role for mucus and sIgA. Antibodies responsible for adhesion inhibition were cross-reactive among several Campylobacter strains and were not directed solely against flagellar antigens.
开发了一种体外黏液测定法，以研究黏液凝胶和分泌型免疫球蛋白A（sIgA）在防止空肠弯曲菌附着于INT 407细胞中的作用。发现兔小肠黏液覆盖层会阻碍空肠弯曲菌附着于INT 407细胞单层。发现来自先前定殖有空肠弯曲菌的兔的黏液能完全抑制细菌黏附于下层细胞。在先前暴露的兔的黏液样本中很容易检测到抗弯曲菌sIgA，并且它负责消除细菌对INT 407细胞的黏附。用弯曲菌细胞吸收黏液后抑制作用消失，证明了这一点。含sIgA的黏液在测定系统的黏液层内导致空肠弯曲菌细胞聚集。单独的非免疫黏液和sIgA无法引起细菌聚集，这表明黏液和sIgA具有协同作用。负责黏附抑制的抗体在几种弯曲菌菌株之间具有交叉反应性，并且并非仅针对鞭毛抗原。
