Department of Genetics, Development and Cell Biology, Iowa State University, Ames, Iowa, USA.
Zebrafish. 2022 Jun;19(3):104-108. doi: 10.1089/zeb.2021.0082.
Cleavage Under Targets and Release Using Nuclease (CUT&RUN) has emerged as a chromatin profiling strategy that excels traditional methods. Although CUT&RUN has been widely utilized in mammalian cells, its use in the zebrafish is at its early stages. In this study, we have developed a protocol to successfully perform CUT&RUN to map transcription factor (TF) binding sites in embryonic, adult tissues, and FACS-sorted zebrafish cells. We also provide a detailed workflow for the identification of predicted TF binding sites that can be utilized in any animal species. Altogether, our strategy will expand this invaluable tool to the zebrafish community, improving the epigenetic resolution that can be achieved in this model organism.
靶向切割和核酸酶释放（CUT&RUN）技术已经成为一种优于传统方法的染色质分析策略。尽管 CUT&RUN 已被广泛应用于哺乳动物细胞，但在斑马鱼中的应用还处于早期阶段。在本研究中，我们开发了一种成功进行 CUT&RUN 的方案，以绘制胚胎、成年组织和 FACS 分选的斑马鱼细胞中转录因子（TF）结合位点图谱。我们还提供了一个详细的工作流程，用于鉴定可用于任何动物物种的预测 TF 结合位点。总之，我们的策略将把这个非常有价值的工具扩展到斑马鱼研究领域，提高该模式生物中可实现的表观遗传学分辨率。
