Karoui Hedi, Patwal Pankaj Singh, Pavan Kumar B V V S, Martin Nicolas
Univ. Bordeaux, CNRS, Centre de Recherche Paul Pascal, UMR 5031, Pessac, France.
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee, India.
Front Mol Biosci. 2022 May 26;9:880525. doi: 10.3389/fmolb.2022.880525. eCollection 2022.
In the past decade, the focus of bottom-up synthetic biology has shifted from the design of complex artificial cell architectures to the design of interactions between artificial cells mediated by physical and chemical cues. Engineering communication between artificial cells is crucial for the realization of coordinated dynamic behaviours in artificial cell populations, which would have implications for biotechnology, advanced colloidal materials and regenerative medicine. In this review, we focus our discussion on molecular communication between artificial cells. We cover basic concepts such as the importance of compartmentalization, the metabolic machinery driving signaling across cell boundaries and the different modes of communication used. The various studies in artificial cell signaling have been classified based on the distance between sender and receiver cells, just like in biology into autocrine, juxtacrine, paracrine and endocrine signaling. Emerging tools available for the design of dynamic and adaptive signaling are highlighted and some recent advances of signaling-enabled collective behaviours, such as quorum sensing, travelling pulses and predator-prey behaviour, are also discussed.
在过去十年中，自下而上的合成生物学重点已从复杂人工细胞架构的设计转向由物理和化学信号介导的人工细胞间相互作用的设计。人工细胞间的工程通信对于在人工细胞群体中实现协调的动态行为至关重要，这将对生物技术、先进胶体材料和再生医学产生影响。在本综述中，我们将讨论重点放在人工细胞间的分子通信上。我们涵盖了诸如区室化的重要性、驱动跨细胞边界信号传导的代谢机制以及所使用的不同通信模式等基本概念。人工细胞信号传导的各种研究已根据发送细胞和接收细胞之间的距离进行分类，就像在生物学中分为自分泌、旁分泌、近分泌和内分泌信号传导一样。文中突出了可用于设计动态和适应性信号传导的新兴工具，并讨论了一些具有信号传导功能的集体行为的最新进展，如群体感应、传播脉冲和捕食者 - 猎物行为。
