Medicinal and Aromatic Plants Research Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Giza, 12622, Egypt.
Pharmacology Department, Medicine and Clinical Studies Research Institute, National Research Centre, Dokki, Giza, 12622, Egypt.
Sci Rep. 2022 Jun 22;12(1):10595. doi: 10.1038/s41598-022-14599-8.
Musa acuminata (MA) is a popular fruit peels in the world. Non-food parts of the plant have been investigated for their antioxidant and anti-ulcerative colitis activity. Metabolomic approaches were found to be informative as a screening tool. It discovered different metabolites depending on statistical analysis. The antioxidant activity content was measured by colorimetric method. Seventy six investigated metabolites were observed. The identities of some of these markers were confirmed based on their MS fragmentation and NMR spectroscopy. These include: cinnamic acid and its dimer 2-hydroxy-4-(4-methoxyphenyl)-1H-phenalen-1-one beside; gallic acid and flavonoids; quercetin, quercetin-3-O-β-D-glucoside, luteolin-7-O-β-D-glucopyranoside. GC/MS analysis of MA peels essential oil led to identification of 37 compounds. The leaves, pseudostem and fruit peels extracts were tested for their safety and their anti-ulcerative colitis efficacy in rats. Rats were classified into: normal, positive, prednisolone reference group, MA extracts pretreated groups (250-500 mg/kg) for 2 weeks followed by induction of ulcerative colitis by per-rectal infusion of 8% acetic acid. Macroscopic and microscopic examinations were done. Inflammatory markers (ANCA, CRP and Ilβ6) were measured in sera. The butanol extracts showed good antioxidant and anti-inflammatory activities as they ameliorated macroscopic and microscopic signs of ulcerative colitis and lowered the inflammatory markers compared to untreated group. MA wastes can be a potential source of bioactive metabolites for industrial use and future employment as promising anti-ulcerative colitis food supplements.
食用蕉（Musa acuminata）是一种受欢迎的水果皮，其植物的非食用部分已被研究具有抗氧化和抗溃疡性结肠炎活性。代谢组学方法被发现是一种有用的筛选工具，可以根据统计分析发现不同的代谢物。抗氧化活性含量通过比色法测量。观察到 76 种被调查的代谢物。其中一些标志物的鉴定是基于它们的 MS 碎片和 NMR 光谱。这些标志物包括：肉桂酸及其二聚体 2-羟基-4-（4-甲氧基苯基）-1H-菲伦-1-酮；没食子酸和类黄酮；槲皮素、槲皮素-3-O-β-D-葡萄糖苷、木樨草素-7-O-β-D-葡萄糖苷。食用蕉果皮精油的 GC/MS 分析导致了 37 种化合物的鉴定。对叶子、假茎和果皮提取物进行了安全性测试，并在大鼠中测试了它们的抗溃疡性结肠炎功效。大鼠分为：正常组、阳性组、泼尼松龙参考组、食用蕉提取物预处理组（250-500mg/kg）2 周，然后通过直肠内输注 8%乙酸诱导溃疡性结肠炎。进行了宏观和微观检查。在血清中测量了炎症标志物（ANCA、CRP 和 Ilβ6）。丁醇提取物具有良好的抗氧化和抗炎活性，因为它们改善了溃疡性结肠炎的宏观和微观症状，并降低了与未处理组相比的炎症标志物。食用蕉废物可以作为工业用途和未来作为有前途的抗溃疡性结肠炎食品补充剂的生物活性代谢物的潜在来源。
