马输卵管类器官的生成与冷冻保存。

Thompson Riley E, Meyers Mindy A, Veeramachaneni D N Rao, Pukazhenthi Budhan S, Hollinshead Fiona K

Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USA.

Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.

Methods Protoc. 2022 Jun 15;5(3):51. doi: 10.3390/mps5030051.

Organoids are a type of three-dimensional (3D) cell culture that more closely mimic the in vivo environment and can be maintained in the long term. To date, oviductal organoids have only been reported in laboratory mice, women, and cattle. Equine oviductal organoids were generated and cultured for 42 days (including 3 passages and freeze-thawing at passage 1). Consistent with the reports in mouse and human oviductal organoids, the equine oviductal organoids revealed round cell clusters with a central lumen. Developing a 3D model of the mare oviduct may allow for an increased understanding of their normal physiology, including hormonal regulation. These organoids may provide an environment that mimics the in vivo equine oviduct and facilitate improved in vitro embryo production in equids.

类器官是一种三维（3D）细胞培养物，能更紧密地模拟体内环境且可长期维持。迄今为止，仅在实验室小鼠、女性和牛中报道过输卵管类器官。马的输卵管类器官已被生成并培养了42天（包括3次传代以及第1代时的冻融）。与小鼠和人类输卵管类器官的报道一致，马的输卵管类器官显示出带有中央管腔的圆形细胞簇。建立母马输卵管的3D模型可能有助于增进对其正常生理学的理解，包括激素调节。这些类器官可能提供一个模拟体内马输卵管的环境，并有助于改进马属动物的体外胚胎生产。