Zhuo Haiwei, Zhang Xi, Li Maogen, Zhang Qian, Wang Yonglan
School and Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China.
Antibiotics (Basel). 2022 Jun 1;11(6):754. doi: 10.3390/antibiotics11060754.
Peri-implantitis is a pathological condition involving tissues around dental implants that are characterized by inflammation of the peri-implant mucosa and progressive loss of supporting bone. We found that the antimicrobial peptide KR-12-3 (KRIVKWIKKFLR) derived from LL-37 had antibacterial properties against . The purpose of this study was to evaluate its antibacterial and anti-inflammatory activities and its underlying mechanisms. We evaluated the antibacterial activities of antimicrobial peptides in planktonic and biofilm states by measuring their minimum inhibitory concentration, minimum bactericidal concentration, and biofilm susceptibility. The effects of antimicrobial peptides on the production of IL-6 and IL-8 in LPS-stimulated RAW264.7 cells were detected by enzyme-linked immunosorbent assay and other experiments, and their toxicity to MC3T3-E1 cells was also studied. While maintaining low cytotoxicity, KR-12-3 exhibited growth inhibitory effects on in planktonic and biofilm states. Lower concentrations of KR-12-3 treatment reduced the production of inflammatory cytokines in LPS-stimulated RAW264.8 cells. The mechanisms underlying the inhibition of biofilm formation and anti-inflammatory effects have been associated with the low expression of related genes. KR-12-3 may be used to develop an antibacterial, anti-infective, and anti-inflammatory drugs for peri-implantitis.
种植体周围炎是一种涉及牙种植体周围组织的病理状况，其特征为种植体周围黏膜炎症和支持骨的渐进性丧失。我们发现，源自LL-37的抗菌肽KR-12-3（KRIVKWIKKFLR）对……具有抗菌特性。本研究的目的是评估其抗菌和抗炎活性及其潜在机制。我们通过测量抗菌肽的最低抑菌浓度、最低杀菌浓度和生物膜敏感性，评估了其在浮游菌和生物膜状态下的抗菌活性。通过酶联免疫吸附测定法和其他实验检测了抗菌肽对脂多糖刺激的RAW264.7细胞中白细胞介素-6和白细胞介素-8产生的影响，并研究了其对MC3T3-E1细胞的毒性。在保持低细胞毒性的同时，KR-12-3对浮游菌和生物膜状态下的……均表现出生长抑制作用。较低浓度的KR-12-3处理可降低脂多糖刺激的RAW264.8细胞中炎性细胞因子的产生。生物膜形成抑制和抗炎作用的潜在机制与相关基因的低表达有关。KR-12-3可用于开发治疗种植体周围炎的抗菌、抗感染和抗炎药物。
