Mouzié Cédric M, Guefack Michel-Gael F, Kianfé Boris Y, Serondo Héritier U, Ponou Beaudelaire K, Siwe-Noundou Xavier, Teponno Rémy B, Krause Rui W M, Kuete Victor, Tapondjou Léon A
Department of Chemistry, Faculty of Science, University of Dschang, Dschang P.O. Box 67, Cameroon.
Department of Biochemistry, Faculty of Science, University of Dschang, Dschang P.O. Box 67, Cameroon.
Pharmaceuticals (Basel). 2022 Jun 7;15(6):725. doi: 10.3390/ph15060725.
Microbial infections are leading causes of death and morbidity all over the world due to the development of the resistance to antibiotics by certain microorganisms. In this study, the chemical exploration of the ethanol (EtOH) extract of the aerial part of (Dracaenaceae) led to the isolation of one previously unreported chalcone derivative, i.e., 2',4'-dihydroxy-2,3'-dimethoxychalcone (), together with 12 known compounds: 8-()-methylquercetagetin-3,6,3'-trimethyl ether (), methylgalangine (), quercetin (), kaempferol (), 6,8-dimethylchrysin (), ombuine-3--rutinoside (4',7-dimethylquercetin-3---L-rhamnopyranosyl-(1 → 6) --D-glucopyranoside) (), alliospiroside A (), -sitosterol 3--glucopyranoside (), ishigoside (), betulinic acid (), oleanolic acid (), and lupeol (). The structures were determined by spectroscopic and spectrometric analysis including 1- and 2-Dimensional Nuclear Magnetic Resonance (1D- and 2D-NMR), High-Resolution Electrospray Ionization Mass Spectrometry (HRESIMS), and comparison with literature data. The isolated secondary metabolites and crude extract displayed antibacterial activity against some multidrug-resistant strains with minimal inhibitory concentration (MIC) values ranging from 32 to 256 μg/mL. The antibacterial activity of compound against ATCC13048 (MIC value: 32 μg/mL) was higher than that of chloramphenicol used as the reference drug (MIC = 64 μg/mL).
由于某些微生物对抗生素产生耐药性，微生物感染是全球死亡和发病的主要原因。在本研究中，对龙血树科植物地上部分的乙醇提取物进行化学探索，分离得到一种此前未报道的查尔酮衍生物，即2',4'-二羟基-2,3'-二甲氧基查尔酮，以及12种已知化合物：8-（）-甲基槲皮万寿菊素-3,6,3'-三甲醚、甲基高良姜素、槲皮素、山奈酚、6,8-二甲基白杨素、奥布因-3--芸香糖苷（4',7-二甲基槲皮素-3---L-鼠李吡喃糖基-(1→6)--D-葡萄糖吡喃糖苷）、蒜螺苷A、β-谷甾醇3--葡萄糖吡喃糖苷、石胡荽苷、桦木酸、齐墩果酸和羽扇豆醇。通过光谱和质谱分析确定结构，包括一维和二维核磁共振（1D-和2D-NMR）、高分辨率电喷雾电离质谱（HRESIMS），并与文献数据进行比较。分离得到的次生代谢产物和粗提物对一些多重耐药菌株具有抗菌活性，最低抑菌浓度（MIC）值在32至256μg/mL之间。化合物对ATCC13048（MIC值：32μg/mL）的抗菌活性高于用作参考药物的氯霉素（MIC = 64μg/mL）。
