Qiu Dan, Huang Jiang, Guo Guanghao, Hu Jinghuang, Li Yahui, Zhang Hongjun, Liu Hongwei, Yang Li, Zhou Yang, Yang Benzhou, Zhang Yudan, Liu Zhiyong, Li Hongjie
The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
School of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin, China.
Front Plant Sci. 2022 Jun 8;13:918559. doi: 10.3389/fpls.2022.918559. eCollection 2022.
Wheat genotypes resistant to powdery mildew ( f. sp. , ) provide a sustainable means for disease control. We developed a pair of near-isogenic lines H962R and H962S with contrasting reactions to powdery mildew from a residue heterozygous line. H962R was resistant to 127 out of the 136 isolates collected from the major wheat-producing regions of China and showed a similar virulence/avirulence pattern as Fuzhuang 30, Xiaobaidong, and Hongquanmang carrying resistance allele of , but H962S was resistant to none of them. A dominant gene was responsible for the powdery mildew resistance of H962R as revealed by the genetic analysis using segregating populations derived from a cross between H962R and H962S. Molecular marker analysis detected a resistance locus, designated , on a genetic interval of the chromosome arm 7BL where resides. This locus was co-segregated with the functional marker of . The PCR-based sequence alignment of demonstrated that H962R had an identical sequence as Fuzhuang 30 (haplotype HapGA), and H962S possessed the same sequence as the powdery mildew susceptible cultivar Kenong 199. The genomic compositions of lines H962R and H962S were highly comparable as evidenced by only a small percentage of SNP variations detected by the 16K Genotyping by Target Sequencing (GBTS) SNP array and the 90K Illumina iSelect Wheat SNP array. The two lines performed similarly in the yield-related and plant growth traits investigated, except for greater kernel weight in H962R than in H962S. This indicates that has no deleterious effect and can be served as an excellent disease resistance gene in wheat breeding.
抗白粉病的小麦基因型为病害控制提供了一种可持续的方法。我们从一个残余杂合系中培育出了一对对白粉病反应截然不同的近等基因系H962R和H962S。H962R对从中国主要小麦产区收集的136个分离株中的127个具有抗性，并且与携带抗性等位基因的福壮30、小白冬和红泉芒表现出相似的毒力/无毒力模式，但H962S对这些分离株均无抗性。通过对H962R和H962S杂交衍生的分离群体进行遗传分析，结果表明一个显性基因决定了H962R对白粉病的抗性。分子标记分析在7BL染色体臂的遗传区间上检测到一个抗性位点，命名为，该位点与的功能标记共分离。基于PCR的序列比对表明，H962R与福壮30具有相同的序列（单倍型HapGA），而H962S与感白粉病品种科农199具有相同的序列。通过16K靶向测序基因分型（GBTS）SNP芯片和90K Illumina iSelect小麦SNP芯片检测到的SNP变异比例很小，这证明H962R和H962S品系的基因组组成高度相似。除了H962R的粒重高于H962S外，这两个品系在产量相关和植株生长性状方面表现相似。这表明没有有害影响，可作为小麦育种中优良的抗病基因。
