Beijing Key Laboratory for Bioengineering and Sensing Technology, Daxing Research Institute, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization, Weifang University of Science and Technology, Shouguang, China.
Food Res Int. 2022 Jul;157:111256. doi: 10.1016/j.foodres.2022.111256. Epub 2022 Apr 18.
Foodborne diseases caused by foodborne pathogens lead to serious harm to food safety and human health. This work fabricated an enzyme-responsive packaging film based on porous poly (lactic acid) (PLA) nanofibers modified by positively charged polyethyleneimine (PEI) and further to adsorb negatively charged pectin coating, which loaded with thymol (THY) for protecting fruits from microbial infection. The porous PLA nanofibers were fabricated by combining "Breath Figure" principle and electrospinning technique. The XPS and FTIR characterizations showed that PLA nanofiber membrane was successfully modified by PEI. The prepared nanofiber membrane significantly inhibited the growth of E. coli, S. aureus and Bacillus subtilis (＞95%), especially showed excellent antifungal activity against Aspergillus niger. The release of THY from pectin-coated porous nanofiber membrane (THY@PLA-PEI-Pectin) was triggered by pectinase, which was secreted by microorganisms from food contamination. Besides, the biocompatible THY@PLA-PEI-Pectin nanofiber membrane prolonged the shelf life of citrus. Therefore, this pectinase-responsive nanofiber membrane has desirable application prospects in the development of active or smart packaging systems.
食源性病原体引起的食源性疾病对食品安全和人类健康造成严重危害。本工作制备了一种基于带正电荷的聚乙烯亚胺（PEI）改性的多孔聚乳酸（PLA）纳米纤维的酶响应包装膜，并进一步吸附带负电荷的果胶涂层，该涂层负载百里香酚（THY），以保护水果免受微生物感染。多孔 PLA 纳米纤维是通过结合“呼吸图”原理和静电纺丝技术制备的。XPS 和 FTIR 表征表明，PEI 成功地修饰了 PLA 纳米纤维膜。所制备的纳米纤维膜对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌的生长有明显的抑制作用（＞95%），对黑曲霉尤其表现出优异的抗真菌活性。果胶涂层多孔纳米纤维膜（THY@PLA-PEI-Pectin）中的 THY 通过微生物从食物污染中分泌的果胶酶触发释放。此外，生物相容的 THY@PLA-PEI-Pectin 纳米纤维膜延长了柑橘的货架期。因此，这种果胶酶响应纳米纤维膜在开发活性或智能包装系统方面具有良好的应用前景。
