Chen Chong-You, Tsai Pei-Hsuan, Lin Ya-Hui, Huang Chien-Yu, Chung Johnson H Y, Chen Guan-Yu
Institute of Biomedical Engineering, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu, 300093, Taiwan.
Department of Electronics and Electrical Engineering, College of Electrical and Computer Engineering, National Yang Ming Chiao Tung University, Hsinchu, 300093, Taiwan.
Mater Today Bio. 2022 Jun 15;15:100326. doi: 10.1016/j.mtbio.2022.100326. eCollection 2022 Jun.
In tissue engineering, foreign body reactions (FBRs) that may occur after the insertion of medical implants are a considerable challenge. Materials currently used in implants are mainly metals that are non-organic, and the lack of biocompatibility and absence of immune regulations may lead to fibrosis after long periods of implantation. Here, we introduce a highly biocompatible hybrid interface of graphene oxide (GO) and collagen type I (COL-I), where the topological nanostructure can effectively inhibit the differentiation of fibroblasts into myofibroblasts. The structure and roughness of this coating interface can be easily adjusted at the nanoscale level through changes in the GO concentration, thereby effectively inducing the polarization of macrophages to the M1 state without producing excessive amounts of pro-inflammatory factors. Compared to nanomaterials or the extracellular matrix as an anti-fibrotic interface, this hybrid bio-interface has superior mechanical strength, physical structures, and high inflammation. Evidenced by inorganic materials such as glass, titanium, and nitinol, GO-COL shows great potential for use in medical implants and cell-material interfaces.
在组织工程中，医疗植入物插入后可能发生的异物反应（FBRs）是一个相当大的挑战。目前植入物中使用的材料主要是无机金属，缺乏生物相容性和免疫调节功能可能导致长期植入后出现纤维化。在此，我们介绍一种氧化石墨烯（GO）和I型胶原蛋白（COL-I）的高度生物相容性混合界面，其拓扑纳米结构可有效抑制成纤维细胞向肌成纤维细胞的分化。通过改变GO浓度，这种涂层界面的结构和粗糙度可在纳米尺度上轻松调节，从而有效诱导巨噬细胞极化为M1状态，而不会产生过量的促炎因子。与作为抗纤维化界面的纳米材料或细胞外基质相比，这种混合生物界面具有卓越的机械强度、物理结构和抗炎性。以玻璃、钛和镍钛诺等无机材料为证，GO-COL在医疗植入物和细胞-材料界面方面具有巨大的应用潜力。
