Tanimoto Yoshihiko, Ohyama Minori, Ito Erika, Akiyoshi Kyoko, Onishi Yuka, Mori Ai, Nomoto Ryohei
Department of Infectious Diseases, Kobe Institute of Health, 4-6-5 Minatojima-nakamachi, Chuo-ku, Kobe, Hyogo, 650-0046, Japan.
Institute for Life and Medical Sciences, Kyoto University, 53 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, 606-8507, Japan.
BMC Res Notes. 2025 Apr 15;18(1):170. doi: 10.1186/s13104-025-07225-z.
Human adenoviruses (HAdVs) cause various diseases, and they frequently undergo recombination. As adenoviruses are susceptible to recombination, whole-genome sequencing and analysis are essential approaches to understanding viral properties. In the present study, we performed whole-genome sequencing to characterize adenoviruses and assess the local epidemic genotypes of adenovirus-related diseases in Kobe, Japan.
Eighty HAdV cell culture-positive strains isolated from clinical specimens in Kobe City, Japan, between 2018 and 2022 were used. Whole-genome sequencing, phylogenetic analysis, and recombination analysis were performed.
Disease-specific HAdV species were detected, with species B and C being the primary species detected for pharyngoconjunctival fever and species F for infectious gastroenteritis. All species B strains belonged to the HAdV-3 genotype. Multiple genotypes were detected in species C, including five strains in which the new genotype, P85H5F5, was identified in pharyngoconjunctival fever specimens.
Whole-genome analysis of HAdV is an important approach not only for understanding local epidemics, but also for monitoring the emergence of recombinant genotypes.
人腺病毒（HAdVs）可引发多种疾病，且频繁发生重组。由于腺病毒易于重组，全基因组测序与分析是了解病毒特性的重要方法。在本研究中，我们进行了全基因组测序，以鉴定腺病毒并评估日本神户腺病毒相关疾病的当地流行基因型。
使用了2018年至2022年间从日本神户市临床标本中分离出的80株HAdV细胞培养阳性菌株。进行了全基因组测序、系统发育分析和重组分析。
检测到特定疾病的HAdV种类，其中B种和C种是咽结膜热检测到的主要种类，F种是感染性肠胃炎检测到的主要种类。所有B种菌株均属于HAdV - 3基因型。在C种中检测到多种基因型，包括在咽结膜热标本中鉴定出新型基因型P85H5F5的5株菌株。
HAdV的全基因组分析不仅是了解当地疫情的重要方法，也是监测重组基因型出现的重要方法。
