Poostchi Ophtalmalogy Research Center, Department of Ophtalmology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Eur J Med Res. 2023 Mar 2;28(1):108. doi: 10.1186/s40001-022-00928-0.
Adenoviral-mediated keratoconjunctivitis is among the emergency diseases of ophthalmology with long-term sequels. The role of adenovirus infection, ocular-related genotypes, and association with ocular symptoms need to be investigated for epidemiological as well as clinical purposes.
The affected patients from two close keratoconjunctivitis epidemics were included in the study. The swab samples were taken from patients; the total DNA was extracted and then used as a template for in-house Real-time PCR. Besides, partial Hexon genes of 11 adenovirus positive samples were amplified and submitted to sanger sequencing. Moreover, they were finally evaluated by phylogenetic analysis.
Of 153 patients, 92 (60.1%) were males and 47 cases (30.7%) had a history of eye infection in the family or colleagues. Real-time PCR tests of 126 samples (82.4%) were positive for adenovirus, and all eleven cases that underwent sequencing analysis were determined to be group 8 (HAdV-D8). Adenovirus infection has a significant relationship with infection among family or colleagues (p = 0.048), membrane formation (p = 0.047), conjunctival bleeding (p = 0.046), tearing, and pain(p < 0.05).
The results indicated that Adenovirus is the major cause of keratoconjunctivitis, and HAdV-D8 was the most common genotype in the area. There were some clinical manifestations associated with Adenovirus infection of the conjunctiva.
腺病毒相关性角结膜炎是眼科急症之一，可导致长期后遗症。为了流行病学和临床目的，需要研究腺病毒感染、与眼相关的基因型以及与眼部症状的关联。
本研究纳入了两起密切相关的角结膜炎流行中的受影响患者。从患者中采集拭子样本，提取总 DNA 作为模板，进行内部实时 PCR。此外，还扩增了 11 个腺病毒阳性样本的部分 Hexon 基因，并提交给 Sanger 测序。最后通过系统发育分析进行评估。
在 153 名患者中，92 名（60.1%）为男性，47 例（30.7%）有家族或同事的眼部感染史。126 份样本（82.4%）的实时 PCR 检测结果为腺病毒阳性，对所有 11 个进行测序分析的样本均确定为 8 组（HAdV-D8）。腺病毒感染与家族或同事感染（p=0.048）、膜形成（p=0.047）、结膜出血（p=0.046）、流泪和疼痛（p<0.05）有显著关系。
结果表明，腺病毒是角结膜炎的主要病因，HAdV-D8 是该地区最常见的基因型。结膜腺病毒感染与一些临床表现有关。
