Luo Dongyuan, Chen Boying, Chen Yu
Guangzhou Women and Children's Medical Center, Guangzhou, China.
Front Bioeng Biotechnol. 2022 Jun 14;10:939199. doi: 10.3389/fbioe.2022.939199. eCollection 2022.
The advances in the field of tissue engineering and regenerative medicine have opened new vistas for the repair of alveolar clefts. However, the currently available biomaterials used for the repair of alveolar clefts have poor mechanical properties and biocompatibility, which hinders the treatment outcomes. Here, we aimed to develop 3D printed biomimetic scaffolds that fuses β-tricalcium phosphate (β-TCP) and bone marrow mesenchymal stem cells (BMSCs) for improving the repair of alveolar clefts. The methacrylate gelatin (GelMA) was mixed with β-TCP for the preparation of GelMA/β-TCP hybrid scaffolds via 3D printing platform and chemically cross-linking with UV light. The physicochemical properties of the hydrogel scaffolds were characterized. Moreover, the survival state, proliferation ability, morphological characteristics, and osteogenic induction of BMSCs were examined. The prepared hybrid scaffolds showed good biocompatibility and mechanical properties. BMSCs attached well to the scaffolds and proliferated, survived, differentiated, and stimulated osteogenesis for the reconstruction of alveolar clefts. We expect that use of the prepared hybrid hydrogel scaffold can improve the outcomes of alveolar cleft repair in clinic and expand the application of hybrid hydrogel in tissue engineering repair.
组织工程和再生医学领域的进展为牙槽嵴裂的修复开辟了新的前景。然而，目前用于牙槽嵴裂修复的生物材料机械性能和生物相容性较差，这阻碍了治疗效果。在此，我们旨在开发一种融合β-磷酸三钙（β-TCP）和骨髓间充质干细胞（BMSCs）的3D打印仿生支架，以改善牙槽嵴裂的修复。将甲基丙烯酸明胶（GelMA）与β-TCP混合，通过3D打印平台制备GelMA/β-TCP混合支架，并通过紫外线进行化学交联。对水凝胶支架的物理化学性质进行了表征。此外，还检测了BMSCs的存活状态、增殖能力、形态特征和成骨诱导情况。制备的混合支架具有良好的生物相容性和机械性能。BMSCs能很好地附着在支架上并增殖、存活、分化，并刺激成骨以重建牙槽嵴裂。我们期望使用制备的混合水凝胶支架能够改善临床牙槽嵴裂修复的效果，并扩大混合水凝胶在组织工程修复中的应用。
