Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad, 500046, India.
Bacterial Discovery Laboratory,Centre for Environment, Institute of Science and Technology, JNT University Hyderabad, Kukatpally, Hyderabad, 500085, India.
Antonie Van Leeuwenhoek. 2021 Apr;114(4):341-353. doi: 10.1007/s10482-020-01515-1. Epub 2021 Feb 13.
A Gram-stain-negative, aerobic, pear to oval shaped, rosette forming bacterium with crateriform structures well distributed on the cell surface designated as strain JC647 was isolated from a sponge specimen belonging to the genus Spongia. Strain JC647 reproduces through budding. Strain JC647 shared highest 16S rRNA gene sequence identity of 99.9% with "Crateriforma conspicua" Mal65 (not a valid species name). The genome size of strain JC647 is 6.9 Mb with a G + C content of 57.8 mol %. For the resolution of the phylogenetic congruence of the novel strain, the phylogeny was also constructed with the sequences of ninety-two housekeeping genes. Based on the phylogenetic analyses, low dDDH value (51.0%), low gANI (93.2%), low AAI (94.9%) results, chemotaxonomic characteristics and differential physiological properties, strain JC647 is recognized as a new species of the genus "Crateriforma", for which we propose the name Crateriforma spongiae sp. nov. The type species is JC647 (= KCTC 72176 = NBRC 114068).
从属于海绵属的海绵标本中分离到一株革兰氏阴性、需氧、呈梨形到椭圆形、具有良好分布的坑状结构的玫瑰形成菌，命名为 JC647 菌株。JC647 菌株通过出芽繁殖。JC647 菌株与“Crateriforma conspicua”Mal65（不是有效种名）的 16S rRNA 基因序列相似度最高，为 99.9%。JC647 菌株的基因组大小为 6.9Mb，GC 含量为 57.8mol%。为了解决新菌株的系统发育一致性问题，还利用 92 个看家基因的序列构建了系统发育树。基于系统发育分析、低 dDDH 值（51.0%）、低 gANI（93.2%）、低 AAI（94.9%）结果、化学生态特征和差异生理特性，JC647 菌株被认定为“Crateriforma”属的一个新种，我们将其命名为 Crateriforma spongiae sp. nov. 模式种为 JC647（=KCTC 72176=NBRC 114068）。
