Chik Mazzura Wan, Mohd Affandi Meor Mohd Redzuan Meor, Singh Gurmeet Kaur Surindar
Department of Pharmaceutical Life Sciences, Faculty of Pharmacy, Universiti Teknologi MARA, Kampus Puncak Alam, Bandar Puncak Alam, Selangor, Malaysia.
Department of Pharmaceutics, Faculty of Pharmacy, Universiti Teknologi MARA, Kampus Puncak Alam, Bandar Puncak Alam, Selangor, Malaysia.
J Pharm Bioallied Sci. 2022 Jan-Mar;14(1):25-30. doi: 10.4103/jpbs.jpbs_464_21. Epub 2022 May 19.
Astaxanthin (Ast), a compound used widely as a dietary supplement, has high antioxidant properties but poor oral bioavailability. To benefit from its nutritional values in cognitive function, Ast was formulated into a nanoemulsion which may improve its penetration through the blood-brain barrier (BBB).
The present study aims to quantitate the Ast nanoemulsion in different regions of the brain tissue using the high-performance liquid chromatography method.
Sprague-Dawley rats were fed with Ast nanoemulsion formulation daily (40, 80, and 160 mg/kg body weight, bw) for 28 days before brain tissues were harvested, extracted, and quantified. A simple, sensitive, and reliable method using high-performance liquid chromatography with an ultraviolent detector was developed and validated to quantify Ast in the brain.
Data were analyzed using the ToolPak Data Analysis in Excel for -test and analysis of variance single factor with Tukey analysis.
The calibration curve demonstrated a linear regression with an of 0.9998 and absolute recovery ranging from 97.8% to 109.6%. The hippocampus of the 160 mg/kg bw treatment group showed a significantly higher concentration of Ast (77.9 ± 17.3 μg/g) compared to the cortex (22.3 ± 4.2 μg/g) and cerebellum (33.1 ± 5.4 μg/g). Ast was detected in the cerebellum of the 80 mg/kg bw (29.4 ± 7.8 μg/g) treatment group with the amount not being significantly different to the 160 mg/kg bw (33.1 ± 5.4 μg/g) treatment group.
It was evident that the Ast nanoemulsion formulated was able to cross the BBB and may provide protective benefits.
虾青素（Ast）是一种广泛用作膳食补充剂的化合物，具有高抗氧化特性，但口服生物利用度较差。为了从其对认知功能的营养价值中获益，虾青素被制成纳米乳剂，这可能会改善其透过血脑屏障（BBB）的能力。
本研究旨在使用高效液相色谱法对脑组织不同区域的虾青素纳米乳剂进行定量分析。
将斯普拉格-道利大鼠每天喂食虾青素纳米乳剂制剂（40、80和160毫克/千克体重，bw），持续28天，然后采集、提取并定量脑组织。开发并验证了一种使用配备紫外检测器的高效液相色谱法的简单、灵敏且可靠的方法，用于定量脑组织中的虾青素。
使用Excel中的ToolPak数据分析工具进行t检验和单因素方差分析，并采用Tukey分析对数据进行分析。
校准曲线显示线性回归，r为0.9998，绝对回收率在97.8%至109.6%之间。与皮质（22.3±4.2微克/克）和小脑（33.1±5.4微克/克）相比，160毫克/千克体重治疗组的海马体中虾青素浓度显著更高（77.9±17.3微克/克）。在80毫克/千克体重治疗组的小脑中检测到虾青素（29.4±7.8微克/克），其含量与160毫克/千克体重治疗组（33.1±5.4微克/克）无显著差异。
显然，所制备的虾青素纳米乳剂能够穿过血脑屏障，并可能提供保护作用。
