Liver and Digestive Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Microbiology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Mol Biol Rep. 2022 Sep;49(9):8827-8834. doi: 10.1007/s11033-022-07732-3. Epub 2022 Jul 7.
Helicobacter pylori as the causative agent of the most common chronic bacterial infectious disease in human still involves a range of clinical challenging complications. In this meantime, the survey of the interaction between H. pylori virulence genes expression and its consequences on gastric antral epithelial cells is Controversial. This study surveyed the correlations between H. pylori cag Pathogenicity Island and virulence factors genes with Fgf7 gene expression as an angiogenic factor in developing gastric cancer in gastric antral epithelial cells of patients with H. pylori infection.
Gastric antral biopsy samples collected from patients out of exclusion criteria, including consumption of tobacco, alchohol and anti-H. pylori drugs, were categorized into gastric cancer (case group n:53) and gastritis (control group n:50) with and without H. pylori infection to detect changes in cDNA of fgf7 in gastric antral epithelial cells by using Real Time RT PCR. Extracted total RNA from gastric antral biopsy samples was used to synthesize cDNA for real time PCR. Furthermore, the cDNA of H. pylori cag Pathogenicity Island and other virulence factors genes were detected by using specific designed primers and simple PCR.
Fgf7 gene expression revealed a significantly increase in gastric antral epithelial cells of gastric cancer and H. pylori-positive patients in contrast with gastritis and H. pylori-negative patients (p < 0.05). In the meanwhile, cag Pathogenicity Island and hopQ genotypes showed a positive correlation with Fgf7 gene expression (fold changes of cDNA) in gastric antral epithelial cells (p < 0.05).
This study revealed an obvious correlation between Fgf7 gene expression in gastric antral epithelial cells of patients with H. pylori carcinogenic genotypes infection and some host factors including age.
幽门螺杆菌作为人类最常见的慢性细菌性传染病的病原体，仍然涉及一系列具有临床挑战性的并发症。与此同时，幽门螺杆菌毒力基因表达与其对胃窦上皮细胞的影响之间的相互作用的调查结果存在争议。本研究调查了幽门螺杆菌 cag 致病岛与毒力因子基因与作为胃癌发生中血管生成因子的 Fgf7 基因表达之间的相关性，在感染幽门螺杆菌的患者的胃窦上皮细胞中。
从符合排除标准（包括吸烟、饮酒和抗幽门螺杆菌药物）的患者中收集胃窦活检样本，分为胃癌（病例组 n：53）和胃炎（对照组 n：50），包括有和没有幽门螺杆菌感染，以检测 Real Time RT-PCR 中胃窦上皮细胞中 fgf7 的 cDNA 变化。从胃窦活检样本中提取总 RNA 用于实时 PCR 的 cDNA 合成。此外，使用特异性设计的引物和简单 PCR 检测幽门螺杆菌 cag 致病岛和其他毒力因子基因的 cDNA。
与胃炎和无幽门螺杆菌感染的患者相比，胃癌和幽门螺杆菌阳性患者的胃窦上皮细胞中 Fgf7 基因表达明显增加（p<0.05）。与此同时，cag 致病岛和 hopQ 基因型与胃窦上皮细胞中 Fgf7 基因表达（cDNA 倍数变化）呈正相关（p<0.05）。
本研究揭示了感染具有致癌基因型的幽门螺杆菌的患者胃窦上皮细胞中 Fgf7 基因表达与一些宿主因素（包括年龄）之间存在明显相关性。
