Hashida Noriyasu, Asao Kazunobu, Hara Chikako, Quantock Andrew J, Saita Ryotaro, Kurakami Hiroyuki, Maruyama Kazuichi, Nishida Kohji
Department of Ophthalmology, Osaka University Graduate School of Medicine, Osaka, Japan.
Structural Biophysics Group, School of Optometry and Vision Sciences, Cardiff University, Cardiff, United Kingdom.
Front Med (Lausanne). 2022 Jun 21;9:938600. doi: 10.3389/fmed.2022.938600. eCollection 2022.
The literature suggests that stress may play a pivotal role in the precipitation of acute central serous chorioretinopathy (CSC) because chorioretinal integrity can be affected by the psychosocial state of the patient, indicating the need for a biomarker. Not only physical stress but also psychological stress causes many types of physical disorders. However, little is known about the pathophysiology of stress-induced disease. The objective of this study was to investigate whether serum factors might be involved in the development of stress-induced ocular diseases.
This observational case series included 33 eyes of 33 consecutive patients with treatment-naïve acute CSC. Fifty eyes of 50 age-matched healthy volunteers were included in this study as non-CSC controls. Serum samples were collected from all participants, and the levels of mitochondrial DNA (mtDNA) were measured by quantitative real-time (RT)-PCR. Serum levels of high-mobility group box (HMGB) 1 and 8-hydroxy-2'-deoxyguanosine (8-OHdG), biological markers of acute/chronic inflammation and oxidative stress, were also measured. The relationships between serum mtDNA, 8-OHdG, and HMGB1 concentrations were investigated by multivariate regression analysis, alongside an assessment of clinical data.
In the treatment-naïve acute CSC group, the serum mtDNA levels (36.5 ± 32.4 ng/mL) were significantly higher than the levels in the control group (7.4 ± 5.9 ng/mL; < 0.001). Serum levels of 8-OHdG and HMGB1 in treatment-naïve acute CSC patients measured 0.12 ± 0.08 ng/mL and 18.1 ± 35.0 ng/mL, respectively, indicating that HMGB1 levels were elevated in CSC compared with the control group. Multivariable regression analysis demonstrated that increased serum mtDNA levels were significantly associated with the height of serous retinal detachment.
We showed serum mtDNA and HMGB1 level elevation and its relation to the clinical activities of CSC, indicating that serum mtDNA and HMGB1 could serve as biomarkers for the acute phase of the disease. The use of these biomarkers makes it possible to predict disease onset and determine disease severity.
文献表明，压力可能在急性中心性浆液性脉络膜视网膜病变（CSC）的发病中起关键作用，因为脉络膜视网膜的完整性会受到患者心理社会状态的影响，这表明需要一种生物标志物。不仅身体压力，心理压力也会引发多种身体疾病。然而，关于压力诱导疾病的病理生理学知之甚少。本研究的目的是调查血清因子是否参与压力诱导性眼部疾病的发生发展。
本观察性病例系列纳入了33例未经治疗的急性CSC患者的33只眼。50例年龄匹配的健康志愿者的50只眼作为非CSC对照组纳入本研究。采集所有参与者的血清样本，通过定量实时（RT）-PCR测量线粒体DNA（mtDNA）水平。还测量了血清高迁移率族蛋白盒（HMGB）1和8-羟基-2'-脱氧鸟苷（8-OHdG）的水平，这两种物质是急性/慢性炎症和氧化应激的生物标志物。通过多变量回归分析研究血清mtDNA、8-OHdG和HMGB1浓度之间的关系，并评估临床数据。
在未经治疗的急性CSC组中，血清mtDNA水平（36.5±32.4 ng/mL）显著高于对照组（7.4±5.9 ng/mL；P<0.001）。未经治疗的急性CSC患者的血清8-OHdG和HMGB1水平分别为0.12±0.08 ng/mL和18.1±35.0 ng/mL，表明与对照组相比，CSC患者的HMGB1水平升高。多变量回归分析表明，血清mtDNA水平升高与浆液性视网膜脱离的高度显著相关。
我们发现血清mtDNA和HMGB1水平升高及其与CSC临床活动的关系，表明血清mtDNA和HMGB1可作为该疾病急性期的生物标志物。使用这些生物标志物可以预测疾病发作并确定疾病严重程度。
