Zhao Zhe-Han, Ju Xian-Yan, Wang Kui-Wu, Chen Xin-Juan, Sun Hong-Xiang, Cheng Ke-Jun
School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China.
Hangzhou Fuchun No.9 Primary School, Hangzhou 311400, China.
Foods. 2022 Jun 22;11(13):1834. doi: 10.3390/foods11131834.
(L.) Kuhn (Pteridaceae family) has been widely used as a food and medicine in China and Korea. Previous studies indicate that contains a variety of bioactive chemical components such as flavonoids, phenols, terpenoids, saponins, polysaccharides, and so on. In the present study, a novel polysaccharide (named as PAP-3) with average molecular weight of 2.14 × 10 Da was obtained from . The structure was studied through physicochemical and spectroscopic analysis. The results indicated that PAP-3 consists of arabinose, rhamnose, fucose, galactose, mannose, and xylose in a molar ratio of 1.58:1.00:3.26:4.57:4.81:3.33. The polysaccharide is mainly composed of (1→2)-linked xylose and (1→3,6)-linked mannose on the main chain, with (1→2)-linked xylose, (1→6)-linked mannose, and (1→6)- and (1→3,6)-linked galactose as side chains. Galactose, fucose, and xylose are located at the end of the side chains. The in vitro immunomodulatory and antioxidant activities were assayed. PAP-3 has strong free-radical scavenging activity on DPPH and ABTS radicals and significant immunomodulatory activity on RAW264.7 cells. These data provide useful information for further study on the polysaccharides of and their applications in the food and medical industries.
(L.) 库恩（凤尾蕨科）在中国和韩国被广泛用作食品和药物。先前的研究表明，其含有多种生物活性化学成分，如黄酮类、酚类、萜类、皂苷、多糖等。在本研究中，从（此处原文未明确提取多糖的植物名称，翻译按原文保留）中获得了一种平均分子量为2.14×10 Da的新型多糖（命名为PAP - 3）。通过物理化学和光谱分析对其结构进行了研究。结果表明，PAP - 3由阿拉伯糖、鼠李糖、岩藻糖、半乳糖、甘露糖和木糖组成，摩尔比为1.58:1.00:3.26:4.57:4.81:3.33。该多糖主链主要由(1→2)连接的木糖和(1→3,6)连接的甘露糖组成，侧链有(1→2)连接的木糖、(1→6)连接的甘露糖以及(1→6)和(1→3,6)连接的半乳糖。半乳糖、岩藻糖和木糖位于侧链末端。测定了其体外免疫调节和抗氧化活性。PAP - 3对DPPH和ABTS自由基具有较强的自由基清除活性，对RAW264.7细胞具有显著的免疫调节活性。这些数据为进一步研究（此处原文未明确提取多糖的植物名称，翻译按原文保留）多糖及其在食品和医药行业的应用提供了有用信息。
