The present study aimed to investigate the impact of a basketball competition on the pre-competitive anxiety and Heart Rate Variability (HRV) of young female basketball players. A total of 12 female basketball players participated in this cross-sectional study. Girls had a mean age of 14 (1.41) years old and a mean experience of 4 (0.85) years practicing basketball. The pre-competitive anxiety and the HRV was assessed the week before and immediately before and after the match. Results showed a significant reduction (p-value < 0.05) of the several HRV variables after the match compared to baseline and pre-competition measures. However, differences between baseline and pre-competition were not found. Furthermore, a significant increase in somatic anxiety after the competition was reported. Taking into account HRV, somatic anxiety, and cognitive anxiety results, young female basketball players did not exhibit an increase in pre-competition anxiety. HRV measurements before competition can help coaches and physical trainers to identify female players with higher pre-competitive anxiety and propose intervention to manage it. Future studies should investigate the impact of coaches and parents on the pre-competitive anxiety of female and male basketball players.