Centro de Investigación en Biotecnología, Instituto Tecnológico de Costa Rica (ITCR), Cartago 30101, Costa Rica.
Molecules. 2022 Jul 2;27(13):4265. doi: 10.3390/molecules27134265.
The use of substances or conditions as elicitors can significantly increase the production of secondary metabolites. In this research, the effects of different elicitors on the production of antioxidant secondary metabolites were evaluated in a strain of sp. The elicitors tested were pH changes in different growth phases of the fungus (pH 3, 5.5 and 8), different concentrations of peptone as a nitrogen source (1 g/L and 10 g/L), and the addition of chemical agents to the culture medium (ethanol, growth regulators, and salts). The alkaline pH during the stationary phase and the high availability of nitrogen were effective elicitors, producing cultures with higher antioxidant activity (37.87 g/L and 43.13 g/L dry biomass) although there were no significant differences with other treatments.
使用物质或条件作为诱导剂可以显著增加次生代谢产物的产量。在这项研究中，评估了不同诱导剂对 sp. 菌株次生代谢物抗氧化活性的影响。测试的诱导剂包括真菌不同生长阶段的 pH 值变化（pH 3、5.5 和 8）、不同浓度的蛋白胨作为氮源（1 g/L 和 10 g/L），以及向培养基中添加化学试剂（乙醇、生长调节剂和盐）。在静止期的碱性 pH 值和高氮可用性是有效的诱导剂，产生的培养物具有更高的抗氧化活性（37.87 g/L 和 43.13 g/L 干生物量），尽管与其他处理没有显著差异。
