Saleem Saira, Kashif Muhammad, Maqbool Rizwana, Ahmed Nisar, Arshad Rubina
Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad 38000, Pakistan.
Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture Faisalabad, Faisalabad 38000, Pakistan.
Plants (Basel). 2022 Jun 27;11(13):1701. doi: 10.3390/plants11131701.
One hundred and five (105) bread wheat ( L.) genotypes, including five commercial checks, were screened for stripe rust resistance at seedling and adult plant stages. Seedlings grown under controlled conditions were screened for disease resistance after 12 days concerning disease incidence percentage after inoculation. K-means cluster analysis divided the genotypes into five different classes according to the presence of virulence/avirulence profile, i.e., class 1, 2, 3, 4 and 5. The same set of genotypes was grown under field conditions for adult plant resistance. Data for disease scoring and different yield and yield-related parameters was recorded. A comparison of breeding lines indicated that all studied traits were negatively affected by disease incidence. Further cluster analysis ranked the genotypes into three distinct groups with Group I and III being the most diverse. Thirteen stripe rust resistance lines were identified using seedling and adult plant resistance strategies. Correlation analysis indicated a negative association between stripe rust incidence and yield and yield-related traits, particularly grains per spike, grain weight per spike, thousand-grain weight, and grain yield per plant. These findings suggested that stripe rust resistance negatively affects yield and yield related traits. The breeding programs aiming at the development of high yielding varieties must also focus on stripe rust resistance.
对105个面包小麦（Triticum aestivum L.）基因型（包括5个商业对照品种）进行了苗期和成株期条锈病抗性筛选。在可控条件下培育的幼苗，接种后12天根据发病率百分比筛选抗病性。K均值聚类分析根据毒性/无毒性谱将基因型分为5个不同类别，即第1类、第2类、第3类、第4类和第5类。同一组基因型在田间条件下种植以评估成株抗性。记录了病害评分以及不同产量和产量相关参数的数据。对育种系的比较表明所有研究性状均受到发病率的负面影响。进一步的聚类分析将基因型分为三个不同的组，其中第I组和第III组差异最大。利用苗期和成株期抗性策略鉴定出13个抗条锈病品系。相关性分析表明条锈病发病率与产量及产量相关性状之间呈负相关，尤其是每穗粒数、每穗粒重、千粒重和单株产量。这些结果表明，抗条锈病对产量及产量相关性状有负面影响。旨在培育高产品种的育种计划也必须注重条锈病抗性。
