用于检测活细胞中脱酰酶活性的 ε--L-乳酰赖氨酸的遗传编码。
Genetic encoding of ε--L-lactyllysine for detecting delactylase activity in living cells.
机构信息
State Key Laboratory of Chemical Oncogenomics, Guangdong Provincial Key Laboratory of Chemical Genomics, School of Chemical Biology and Biotechnology, Peking University Shenzhen Graduate School, Shenzhen, 518055, China.
Institute of Chemical Biology, Shenzhen Bay Laboratory, Shenzhen, 518132, China.
出版信息
Chem Commun (Camb). 2022 Jul 28;58(61):8544-8547. doi: 10.1039/d2cc02643k.
Lysine ε--L-lactylation is a newly discovered post-translational modification. Herein we present the genetic encoding of ε--L-lactyllysine in bacterial and mammalian cells, allowing the preparation of site-specifically ε--L-lactylated recombinant proteins and the construction of fluorescent and luminescent probes for detecting delactylases in living cells. Using these probes, we demonstrate sirtuin 1 as a potential delactylase for non-histone proteins.
赖氨酸 ε--L-乳酰化是一种新发现的翻译后修饰。本文在细菌和哺乳动物细胞中提出了 ε--L-乳酰赖氨酸的遗传编码，允许制备定点 ε--L-乳酰化的重组蛋白，并构建用于检测活细胞中脱酰酶的荧光和发光探针。使用这些探针，我们证明了 Sirtuin 1 是一种潜在的非组蛋白脱酰酶。
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