Kaneshige H
Diabetes. 1987 Jul;36(7):822-8. doi: 10.2337/diab.36.7.822.
Susceptibility to infection is assumed to be increased in diabetic patients, although its mechanism is unknown. The purpose of this study was to determine whether glycosylation of circulating immunoglobulins is related to the decrease of antibody activity in diabetic patients. Thirty-five patients with type II (non-insulin-dependent) diabetes and 14 age-matched normal controls were examined. Nonenzymatic glycosylation of serum immunoglobulin G (IgG) in vivo was measured by two different techniques, colorimetry and affinity chromatography. The levels of glycosylated IgG were significantly higher in diabetic patients than in normal controls. To evaluate the antibody activity of glycosylated IgG, anti-streptolysin O (ASO) titers after in vitro glycosylation of IgG and antibody titers before and after in vivo immunization with influenza vaccine were determined. IgG specific for streptolysin O purified by affinity chromatography decreased ASO titers after in vitro glycosylation. In diabetic patients, serum titers of hemagglutinin-inhibiting antibody against influenza viruses 4 wk after initial immunization were significantly lower than those in normal controls. These results indicate that serum IgG in diabetic patients was nonenzymatically glycosylated, and this modification in vivo might be associated with its functional alteration.
尽管其机制尚不清楚，但糖尿病患者被认为感染易感性增加。本研究的目的是确定循环免疫球蛋白的糖基化是否与糖尿病患者抗体活性降低有关。对35例II型（非胰岛素依赖型）糖尿病患者和14例年龄匹配的正常对照者进行了检查。采用比色法和亲和色谱法两种不同技术测定体内血清免疫球蛋白G（IgG）的非酶糖基化。糖尿病患者糖基化IgG水平显著高于正常对照者。为了评估糖基化IgG的抗体活性，测定了IgG体外糖基化后的抗链球菌溶血素O（ASO）滴度以及流感疫苗体内免疫前后的抗体滴度。通过亲和色谱法纯化的针对链球菌溶血素O的IgG在体外糖基化后降低了ASO滴度。在糖尿病患者中，初次免疫4周后血清中抗流感病毒血凝抑制抗体滴度显著低于正常对照者。这些结果表明糖尿病患者血清IgG发生了非酶糖基化，且这种体内修饰可能与其功能改变有关。
