Wolf C R, Hayward I P, Lawrie S S, Buckton K, McIntyre M A, Adams D J, Lewis A D, Scott A R, Smyth J F
Int J Cancer. 1987 Jun 15;39(6):695-702. doi: 10.1002/ijc.2910390607.
Two ovarian cell lines were derived from the ascites of a patient before and after the onset of resistance to chemotherapy involving cis-platinum, chlorambucil and 5-fluorouracil. Characterization of these lines shows them to have various features in common and some significant differences. Cytologically the lines cannot be distinguished and they both contain high concentrations of oestrogen receptor. However, they do differ with respect to their growth characteristics, karyotype, glutathione content and sensitivity to cis-platinum. The karyotypes of the 2 lines show several marker chromosomes in common but the resistant line contained a chromosome 8 and a 17 which were absent from the earlier sensitive line. This suggests a clonal origin with subsequent divergence to a heterogeneous population.
两种卵巢细胞系源自一名患者化疗耐药（涉及顺铂、苯丁酸氮芥和5-氟尿嘧啶）前后的腹水。对这些细胞系的特征分析表明，它们具有多种共同特征和一些显著差异。在细胞学上，这些细胞系无法区分，并且它们都含有高浓度的雌激素受体。然而，它们在生长特性、核型、谷胱甘肽含量和顺铂敏感性方面存在差异。这两种细胞系的核型显示有几条共同的标记染色体，但耐药细胞系含有一条8号染色体和一条17号染色体，而早期敏感细胞系中不存在这两条染色体。这表明它们起源于克隆，随后分化为异质群体。
