Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre Hospital, Hvidovre, Denmark.
Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.
APMIS. 2022 Oct;130(10):612-617. doi: 10.1111/apm.13262. Epub 2022 Aug 10.
In March 2022, we observed samples with a negative fluorescent signal (60.5%, n = 43) for the influenza A matrix gene and a stronger positive signal for subtype A(H3N2). Forty-three samples were positive in InfA (H3N2) (mean Cq 30.9, range 23.9-35.1), and 26 of the 43 samples were negative in InfA matrix (mean Cq 28.0, range 23.2-30.6). Our multiplex test is a laboratory-developed four-target, four-color influenza A reverse-transcription PCR assay targeting the matrix gene, subtypes A(H3N2) and A(H1N1)pdm09. Several samples were negative when retested on commercial influenza Point-of-Care assays. As the matrix gene is a stand-alone target in most commercial diagnostic assays, we caution against false-negative subtype A test results.
2022 年 3 月，我们观察到流感 A 基质基因的荧光信号呈阴性（60.5%，n=43），而 A(H3N2)亚型的信号则更强阳性。43 个样本 InfA (H3N2) 呈阳性（平均 Cq30.9，范围 23.9-35.1），43 个样本中有 26 个 InfA 基质呈阴性（平均 Cq28.0，范围 23.2-30.6）。我们的多重检测是一种实验室开发的四靶、四色流感 A 逆转录 PCR 检测方法，针对基质基因、A(H3N2)和 A(H1N1)pdm09 亚型。一些样本在商业即时检测上重复检测时呈阴性。由于基质基因是大多数商业诊断检测中的独立靶标，我们警告不要出现假阴性的 A 型检测结果。
