Sulzer C R, Jones W L
Appl Microbiol. 1973 Nov;26(5):655-7. doi: 10.1128/am.26.5.655-657.1973.
An indirect hemagglutination test for the diagnosis of leptospirosis is described; the test uses a soluble antigen from serotype patoc to sensitize sheep erythrocytes which are then fixed with glutaraldehyde. Evaluation of this procedure indicates that it is more reliable than the conventional macroscopic agglutination test and, in contrast with both microscopic and macroscopic agglutination tests, is positive only with sera from persons with current leptospiral illness. The test is simple and convenient and sensitized fixed cells may be stored for at least a year. In comparison with the macroscopic and microscopic tests, only a single antigen is required.
本文描述了一种用于诊断钩端螺旋体病的间接血凝试验；该试验使用来自帕托型血清型的可溶性抗原致敏绵羊红细胞，然后用戊二醛固定。对该方法的评估表明，它比传统的宏观凝集试验更可靠，并且与微观和宏观凝集试验不同，仅对患有当前钩端螺旋体病的人的血清呈阳性。该试验简单方便，致敏固定细胞可保存至少一年。与宏观和微观试验相比，仅需要单一抗原。
