Zheng Xiang-Rong, Zhang Mao-Jiao, Shang Xu-Lan, Fang Sheng-Zuo, Chen Feng-Mao
Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, China.
Front Plant Sci. 2021 Jan 18;11:613499. doi: 10.3389/fpls.2020.613499. eCollection 2020.
is an extremely valuable and multifunctional tree species whose leaves have traditionally been used in used in medicine or as a medicinal tea in China. In recent years, anthracnose has been frequently observed on young leaves of in several nurseries located in Jiangsu Province, resulting in great yield and quality losses. To date, no information is available about the prevalence of anthracnose in China. The main purpose of the present study was to characterize the etiology of anthracnose. Phylogenetic analysis of the eight-loci concatenated dataset revealed that all 44 single-spore isolates belonged to three species in the species complex, namely, , , and sensu stricto. Phenotypic features, including the colony appearance and the morphology of conidia, appressoria, and ascospores, were consistent with the phylogenetic grouping. Virulence tests validated that the three species could cause typical symptoms of anthracnose on leaves, similar to those observed in the field. The optimum mycelial growth temperature ranged from 25 to 30°C for all representative isolates, while s. s. isolates exhibited greater tolerance to high temperature (40°C). Fungicide sensitivity assays indicated that all three species were sensitive to tetramycin, which may be a potential alternative for the management of anthracnose. To our knowledge, this study provides the first report of , , and s. s. causing anthracnose in China as well as in the world.
是一种极具价值且多功能的树种，其叶子在中国传统上被用于医药或制成药茶。近年来，在江苏省的几个苗圃中，幼叶上频繁观察到炭疽病，导致产量和质量大幅损失。迄今为止，关于中国炭疽病的流行情况尚无相关信息。本研究的主要目的是鉴定炭疽病的病因。对八个基因座串联数据集的系统发育分析表明，所有44个单孢分离株属于炭疽菌复合种中的三个物种，即、和狭义的。包括菌落外观以及分生孢子、附着胞和子囊孢子形态在内的表型特征与系统发育分组一致。致病性测试证实，这三个炭疽菌物种可在叶片上引起典型的炭疽病症状，与田间观察到的症状相似。所有代表性分离株的最佳菌丝生长温度范围为25至30°C，而狭义的分离株对高温（40°C）表现出更大的耐受性。杀菌剂敏感性测定表明，所有这三个炭疽菌物种对四霉素敏感，这可能是防治炭疽病的一种潜在替代方法。据我们所知，本研究首次报道了、和狭义的在中国以及世界范围内引起炭疽病。
