Li Gailing, Liu Zhenguo, Ren Fang, Shi Huirong, Zhao Qian, Song Yi, Fan Xunjie, Ma Xiaojun, Qin Guijun
Gynecology Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Department of Infectious Diseases, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Front Microbiol. 2022 Jul 1;13:911992. doi: 10.3389/fmicb.2022.911992. eCollection 2022.
The purpose of this study was to elucidate the characteristics of the gut microbiome in patients with Polycystic ovary syndrome (PCOS) and analyze the alterations of fecal fatty acid metabolism, so as to further provide the pathogenesis of PCOS.
Fecal samples from the PCOS group ( = 31) and healthy control group ( = 27) were analyzed by 16S rRNA gene sequencing and untargeted metabolomics. Peripheral venous blood was collected to measure serum inflammation and intestinal permeability. Finally, the correlation analysis of intestinal flora, fecal metabolites, and laboratory indicators was carried out.
Serum D-lactate content in the PCOS group was higher than that in the control group. There was no significant difference in microbial α diversity and β diversity between PCOS patients and healthy controls. Peptostreptococcaceae and Bacteroidales S24-7 group existed significant differences between PCOS patients and healthy controls. Based on linear discriminant analysis selection, 14 genera including , , and were dominant in patients with PCOS, while 4 genera, including (), (), and (), were dominant in healthy controls. Compared with PCOS with Body mass index (BMI) < 24, patients with BMI ≥ 24 have multiple dominant genera including and . Moreover, serum levels of free testosterone and androstenedione were positively correlated with , while total testosterone was negatively correlated with . Additionally, fecal contents of acetic acid and propionic acid in patients with PCOS were significantly higher than those in healthy controls. and were positively correlated with 6 kinds of fatty acids.
Specific intestinal flora fecal fatty acids and serum metabolites may mediate the occurrence and development of PCOS. PCOS patients with different body sizes have specific intestinal flora.
本研究旨在阐明多囊卵巢综合征（PCOS）患者肠道微生物群的特征，并分析粪便脂肪酸代谢的变化，从而进一步为PCOS的发病机制提供依据。
采用16S rRNA基因测序和非靶向代谢组学方法分析PCOS组（n = 31）和健康对照组（n = 27）的粪便样本。采集外周静脉血以检测血清炎症和肠道通透性。最后，对肠道菌群、粪便代谢产物和实验室指标进行相关性分析。
PCOS组血清D-乳酸含量高于对照组。PCOS患者与健康对照者之间的微生物α多样性和β多样性无显著差异。消化链球菌科和拟杆菌S24-7组在PCOS患者与健康对照者之间存在显著差异。基于线性判别分析选择，PCOS患者中包括、和等14个属占优势，而健康对照者中包括（）、（）和（）等4个属占优势。与体重指数（BMI）<24的PCOS患者相比，BMI≥24的患者有多个优势属，包括和。此外，血清游离睾酮和雄烯二酮水平与呈正相关，而总睾酮与呈负相关。另外，PCOS患者粪便中乙酸和丙酸含量显著高于健康对照者。和与6种脂肪酸呈正相关。
特定的肠道菌群、粪便脂肪酸和血清代谢产物可能介导PCOS的发生发展。不同体型的PCOS患者有特定的肠道菌群。
