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洗胃:一种从兔子获取富含IgA的肠道分泌物的简单方法。

Gastric lavage: a simple method to obtain IgA-rich intestinal secretions from the rabbit.

作者信息

Burr D H, Kerner D T, Blanco C S, Bourgeois A L, Wistar R

出版信息

J Immunol Methods. 1987 May 20;99(2):277-81. doi: 10.1016/0022-1759(87)90138-4.

DOI:10.1016/0022-1759(87)90138-4
PMID:3584997
Abstract

A lavage procedure was developed to obtain intestinal secretions from rabbits. The procedure facilitated the repeated monitoring of the intestinal IgA immune response of these animals to enteric infection with Campylobacter jejuni. This non-invasive technique was easily performed, reproducible and yielded consistent levels of IgA from rabbit intestinal secretions. It is anticipated that this procedure will aid in the study of the intestinal immune response of rabbits to other enteric pathogens.

摘要

已开发出一种灌洗程序,用于从兔子获取肠道分泌物。该程序有助于反复监测这些动物对空肠弯曲菌肠道感染的肠道IgA免疫反应。这种非侵入性技术操作简便、可重复,并且能从兔子肠道分泌物中获得稳定水平的IgA。预计该程序将有助于研究兔子对其他肠道病原体的肠道免疫反应。

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引用本文的文献

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Subunit rotavirus vaccine administered parenterally to rabbits induces active protective immunity.经肠胃外途径给兔子接种的轮状病毒亚单位疫苗可诱导产生主动保护性免疫。
J Virol. 1998 Nov;72(11):9233-46. doi: 10.1128/JVI.72.11.9233-9246.1998.
2
Heat shock- and alkaline pH-induced proteins of Campylobacter jejuni: characterization and immunological properties.空肠弯曲菌的热休克和碱性pH诱导蛋白:特性及免疫学性质
Infect Immun. 1994 Oct;62(10):4256-60. doi: 10.1128/iai.62.10.4256-4260.1994.
3
Mucosal and systemic immunity to Campylobacter jejuni in rabbits after gastric inoculation.
兔经胃接种空肠弯曲菌后的黏膜及全身免疫反应
Infect Immun. 1988 Jan;56(1):99-105. doi: 10.1128/iai.56.1.99-105.1988.
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Significance of flagella in colonization resistance of rabbits immunized with Campylobacter spp.
Infect Immun. 1991 Jul;59(7):2259-64. doi: 10.1128/iai.59.7.2259-2264.1991.
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Serologic and mucosal immune response to rotavirus infection in the rabbit model.兔模型中轮状病毒感染的血清学和黏膜免疫反应
J Virol. 1991 May;65(5):2562-71. doi: 10.1128/JVI.65.5.2562-2571.1991.
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Local immune responses to the Campylobacter flagellin in acute Campylobacter gastrointestinal infection.急性弯曲杆菌胃肠道感染中对弯曲杆菌鞭毛蛋白的局部免疫反应。
J Clin Microbiol. 1992 Feb;30(2):509-11. doi: 10.1128/jcm.30.2.509-511.1992.