Department of Hepatobiliary Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, Shaanxi, China.
National and Local Joint Engineering Research Center of Precision Surgery & Regenerative Medicine, The First Affiliated Hospital of Xi'an Jiaotong University, 277 West Yanta Road, Xi'an, 710061, Shaanxi, China.
Sci Rep. 2022 Jul 20;12(1):12445. doi: 10.1038/s41598-022-16794-z.
Magnetic compression anastomosis, also known as magnamosis, is a safe and feasible method for digestive tract anastomosis. However, the pathological process involved in magnamosis of the digestive tract has not been investigated. This study aimed to establish the stages of digestive tract magnamosis in a rat model. Eighty-four Sprague-Dawley albino rats (200-250 g) were randomly divided into 14 groups (n = 6 per group). All rats underwent colonic magnamosis. Starting from postoperative day (POD) 1, one group of rats was sacrificed every other day to obtain the specimens. Burst pressure at the anastomotic site of each specimen was examined. Gross and histological examination of the anastomotic site was performed to establish the stages of the digestive tract magnamosis. Colonic magnamosis was successfully performed in all rats and the mean anastomosis time was 5.62 ± 0.91 min. The postoperative survival rate was 100%. The lowest anastomotic burst pressure was 78.33 ± 3.44 mmHg on POD3. The anastomotic burst pressure gradually increased and stabilized on POD21. Macroscopic and histological examination showed that the anastomotic mucosal and serosal layer did not heal on POD1. The serosal layer of the anastomosis healed by adhesion on POD3, and the mucosal layer began to heal on POD3-11 and was established by POD21. According to the anastomotic bursting pressure, digestive tract magnamosis can be staged into the magnetic maintenance, fragile, strengthening, and stable phases, which on histology correspond to the serosal adhesion formation, serosal healing, mucosal healing, and stereotyping, respectively.
磁吻合术，又称磁贴合术，是一种安全可行的消化道吻合方法。然而，磁吻合术在消化道中的病理过程尚未得到研究。本研究旨在建立大鼠模型中的消化道磁吻合术分期。84 只 Sprague-Dawley 白化大鼠（200-250g）随机分为 14 组（每组 n=6）。所有大鼠均接受结肠磁吻合术。从术后第 1 天（POD）开始，每隔一天处死一组大鼠以获取标本。检查每个标本吻合部位的爆裂压力。对吻合部位进行大体和组织学检查，以建立消化道磁吻合术分期。所有大鼠均成功进行结肠磁吻合术，平均吻合时间为 5.62±0.91 分钟。术后存活率为 100%。吻合部位最低爆裂压力为 POD3 时的 78.33±3.44mmHg。吻合部位的爆裂压力逐渐增加并在 POD21 时稳定。大体和组织学检查显示，吻合部位的黏膜和浆膜层在 POD1 时未愈合。吻合部位的浆膜层在 POD3 时通过粘连愈合，黏膜层在 POD3-11 时开始愈合，并在 POD21 时建立。根据吻合部位的爆裂压力，消化道磁吻合术可分为磁维持、脆弱、强化和稳定期，在组织学上分别对应于浆膜粘连形成、浆膜愈合、黏膜愈合和定型。
