Mitchell T J, Ketley J M, Burdon D W, Candy D C, Stephen J
J Med Microbiol. 1987 May;23(3):211-9. doi: 10.1099/00222615-23-3-211.
Antibody neutralisation and toxin A elution experiments showed that toxin A uptake from rabbit intestinal lumen was a continuous process. The kinetics of the ileal and colonic responses were significantly different; a much longer incubation (4 h) with toxin was required for colon, compared with 45 min for the ileum, to induce fluid accumulation at 12 h. Fluid secretion was induced only when toxin had gained access to deeper tissues, probably achieved by several toxin uptake-tissue damage cycles. Toxin A induced haemorrhage in both ileal and colonic tissues. In ileum, the villus architecture was severely damaged and this gave rise to protein-rich bloody luminal fluid. In the colon, although colonocytes were removed, the basement membrane remained intact; this resulted in a tissue-localised haemorrhage and a protein-low watery ultrafiltered luminal fluid. Toxin A is thus a novel type of histotoxic enterotoxin.
抗体中和及毒素A洗脱实验表明，兔肠腔对毒素A的摄取是一个持续的过程。回肠和结肠反应的动力学有显著差异；与回肠45分钟相比，结肠需要更长时间（4小时）与毒素孵育，才能在12小时时诱导液体蓄积。只有当毒素进入更深层组织时才会诱导液体分泌，这可能是通过几个毒素摄取-组织损伤循环实现的。毒素A在回肠和结肠组织中均诱导出血。在回肠中，绒毛结构严重受损，这导致富含蛋白质的血性肠腔液产生。在结肠中，虽然结肠上皮细胞被清除，但基底膜保持完整；这导致组织局部出血和蛋白质含量低的水样超滤肠腔液。因此，毒素A是一种新型的组织毒性肠毒素。
