Yun Ji-Hyun, Lee June-Young, Kim Pil Soo, Jung Mi-Ja, Bae Jin-Woo
Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1918-1924. doi: 10.1099/ijsem.0.001887. Epub 2017 Jun 14.
Three strains, CBM1T, CBH23 and LAH16T, belonging to the genus Paenibacillus were isolated from the midgut and hindgut of Apis mellifera. Based on 16S rRNA gene sequence analyses and phenotypic characteristics, the three strains represent two novel species. Strains CBM1T and CBH23 formed a group with Paenibacillus puldeungensis CAU 9324T, and strain LAH16T belonged to the Paenibacillusamylolyticus NRRL NRS-290T subgroup of the genus Paenibacillus. The DNA G+C contents of strains CBM1T, CBH23 and LAH16T were 47.7, 48.1 and 46.1 mol%, respectively. The three strains possessed diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as polar lipids. The predominant quinone in the three strains was MK-7, but strains CBM1T and CBH23 contained an additional major quinone, MK-8(H2). While strain LAH16T and most species of the genus Paenibacillus possessed anteiso-C15 : 0, iso-C16 : 0 and C16 : 0 as major cellular fatty acids, strains CBM1T and CBH23 possessed C16 : 0, anteiso-C15 : 0, C18 : 1ω7c and C19 : 0cyclo ω8c. Based on phenotypic, chemotaxonomic and phylogenetic data, strains CBM1T and CBH23 and the strain LAH16T represent novel species in the genus Paenibacillus, for which the names Paenibacillus apis sp. nov. and Paenibacillus intestini sp. nov. are proposed, with CBM1T (=KCTC 33844T=JCM 31620T) and LAH16T (=KCTC 33832T=JCM 31621T) as the type strains, respectively.
从意大利蜜蜂的中肠和后肠中分离出三株属于芽孢杆菌属的菌株，分别为CBM1T、CBH23和LAH16T。基于16S rRNA基因序列分析和表型特征，这三株菌代表两个新物种。菌株CBM1T和CBH23与Puldeungensis芽孢杆菌CAU 9324T形成一个类群，菌株LAH16T属于芽孢杆菌属解淀粉芽孢杆菌NRRL NRS - 290T亚群。菌株CBM1T、CBH23和LAH16T的DNA G + C含量分别为47.7、48.1和46.1 mol%。这三株菌的极性脂质含有双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。这三株菌的主要醌类是MK - 7，但菌株CBM1T和CBH23还含有另一种主要醌类MK - 8(H2)。虽然菌株LAH16T和大多数芽孢杆菌属物种的主要细胞脂肪酸为anteiso - C15 : 0、iso - C16 : 0和C16 : 0，但菌株CBM1T和CBH23的主要细胞脂肪酸为C16 : 0、anteiso - C15 : 0、C18 : 1ω7c和C19 : 0cyclo ω8c。基于表型、化学分类和系统发育数据，菌株CBM1T和CBH23以及菌株LAH16T代表芽孢杆菌属中的新物种，分别提出名为意大利蜜蜂芽孢杆菌（Paenibacillus apis sp. nov.）和肠道芽孢杆菌（Paenibacillus intestini sp. nov.），分别以CBM1T（=KCTC 33844T = JCM 31620T）和LAH16T（=KCTC 33832T = JCM 31621T）作为模式菌株。
