Miranda Cristobal L, Kumbi Yadano, Wu Wenbin, Lee Hyi-Seung, Reed Ralph L, Stevens Jan F
Linus Pauling Institute, Oregon State University, Corvallis, Oregon, 97331.
Department of Pharmaceutical Sciences, Oregon State University, Corvallis, Oregon, 97331.
Nat Prod Commun. 2022 Jul;17(7). doi: 10.1177/1934578x221109426. Epub 2022 Jul 5.
Phytochemicals from the genus, , have been attracting increasing attention due to their potential beneficial effects on human health. species contain various types of phytochemicals such as flavonoids, alkaloids, saponins, terpenoids, coumarins and tannins. In this study, we investigated the phytochemical composition of unhydrolyzed and acid-hydrolyzed extracts of and their bioactivity toward breast cancer MCF-7 cells in vitro. The results revealed that contains phytochemicals consistent with the reported phytochemical composition of this species, with greater amounts of aglycones detected in the hydrolyzed extract. The crude extract of without acid hydrolysis was found to be ineffective in inhibiting the growth of MCF-7 cells at doses below 1000 μg/mL. However, after acid hydrolysis (to mimic gastro-intestinal hydrolysis), the extract became growth-inhibitory to MCF-7 cells as low as 10 μg/mL and the cytotoxicity increased with increasing dose and time of treatment. The results suggest that extracts contain phytochemicals in glycosidic forms whose aglycones are active as anti-proliferative agents toward breast cancer cells in vitro.
来自[属名]属的植物化学物质因其对人类健康的潜在有益作用而受到越来越多的关注。[物种名]物种含有多种类型的植物化学物质，如黄酮类、生物碱、皂苷、萜类、香豆素和单宁。在本研究中，我们研究了[物种名]未水解和酸水解提取物的植物化学成分及其对乳腺癌MCF-7细胞的体外生物活性。结果表明，[物种名]所含的植物化学物质与该[物种名]物种已报道的植物化学成分一致，在水解提取物中检测到的苷元含量更高。发现未经酸水解的[物种名]粗提取物在剂量低于1000μg/mL时对MCF-7细胞的生长没有抑制作用。然而，经过酸水解（模拟胃肠道水解）后，[物种名]提取物在低至10μg/mL时对MCF-7细胞具有生长抑制作用，并且细胞毒性随着处理剂量和时间的增加而增加。结果表明，[物种名]提取物含有糖苷形式的植物化学物质，其苷元在体外作为抗增殖剂对乳腺癌细胞具有活性。
