Faculty of Science & Technology, Meijo University, 1-501 Shiogamaguchi, Tempaku-ku, Nagoya 468-8502, Japan.
Graduate School of Environmental and Human Sciences, Meijo University, Nagoya 468-8502, Japan.
Mar Drugs. 2022 Jun 24;20(7):414. doi: 10.3390/md20070414.
The effect of oral supplementation with astaxanthin of different -isomer ratios on ultraviolet (UV) light-induced skin damage in guinea pigs was investigated. Astaxanthin with a high -isomer content was prepared from the all--isomer via thermal isomerization. Intact (all-)-astaxanthin and the prepared -isomer-rich astaxanthin were suspended in soybean oil and fed to guinea pigs for three weeks. The UV-light irradiation was applied to the dorsal skin on the seventh day after the start of the test diet supplementation, and skin parameters, such as elasticity, transepidermal water loss (TEWL), and pigmentation (melanin and erythema values), were evaluated. The accumulation of astaxanthin in the dorsal skin was almost the same after consumption of the all--isomer-rich astaxanthin diet (E-AST-D; total -isomer ratio = 3.2%) and the -isomer-rich astaxanthin diet (Z-AST-D; total -isomer ratio = 84.4%); however, the total -isomer ratio of astaxanthin in the skin was higher in the case of the Z-AST-D supplementation. Both diets inhibited UV light-induced skin-damaging effects, such as the reduction in elasticity and the increase in TEWL level. Between E-AST-D and Z-AST-D, Z-AST-D showed better skin-protective ability against UV-light exposure than E-AST-D, which might be because of the greater UV-light-shielding ability of astaxanthin -isomers than the all--isomer. Furthermore, supplementation with Z-AST-D resulted in a greater reduction in skin pigmentation caused by astaxanthin accumulation compared to that of E-AST-D. This study indicates that dietary astaxanthin accumulates in the skin and appears to prevent UV light-induced skin damage, and the -isomers are more potent oral sunscreen agents than the all--isomer.
研究了不同异构体比例的虾青素口服补充剂对豚鼠紫外线（UV）光诱导皮肤损伤的影响。通过热异构化，从全异构体中制备出高异构体含量的虾青素。将完整的（全）-虾青素和制备的富含异构体的虾青素悬浮在大豆油中，并喂给豚鼠三周。在开始测试饮食补充后的第七天，将 UV 光照射到背部皮肤，评估皮肤参数，如弹性、经皮水分流失（TEWL）和色素沉着（黑色素和红斑值）。在食用富含全异构体的虾青素饮食（E-AST-D；总异构体比例=3.2%）和富含异构体的虾青素饮食（Z-AST-D；总异构体比例=84.4%）后，背部皮肤中虾青素的积累几乎相同；然而，在 Z-AST-D 补充的情况下，虾青素的总异构体比例更高。这两种饮食都抑制了 UV 光诱导的皮肤损伤作用，如弹性降低和 TEWL 水平升高。与 E-AST-D 相比，Z-AST-D 对 UV 光暴露的皮肤保护能力更强，这可能是因为虾青素异构体比全异构体具有更好的紫外线屏蔽能力。此外，与 E-AST-D 相比，Z-AST-D 补充剂可更大程度地减少虾青素积累引起的皮肤色素沉着。本研究表明，膳食虾青素在皮肤中积累并可预防 UV 光诱导的皮肤损伤，且异构体是比全异构体更有效的口服防晒剂。
