School of Food Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Coastal Agriculture Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea.
J Microbiol Biotechnol. 2022 Aug 28;32(8):976-981. doi: 10.4014/jmb.2206.06037. Epub 2022 Jul 26.
Phenanthrenes are bioactive phenolic compounds found in genus , in which they are distributed more in peel than in flesh. Recent studies on phenanthrenes from sp. peels have revealed the potential for valuable biomaterials. Herein, an analytical method using high-performance liquid chromatography (HPLC) for quantitation of bioactive phenanthrenes was developed and validated. The calibration curves were obtained using the phenanthrenes (1-3) previously isolated from concentrations in the range of 0.625-20.00 μg/ml with a satisfactory coefficient of determination (R) of 0.999. The limit of detection (LOD) and the limit of quantification (LOQ) values of the isolated phenanthrenes ranged from 0.78-0.89 and 2.38-2.71 μg/ml, respectively. The intraday and interday precision ranged from 0.25-7.58%. The recoveries of the isolated phenanthrenes were from 95 to 100% at concentrations of 1.25, 2.5, and 5.0 μg/ml. Additionally, phenanthrenes (1-3) were found in all investigated peel extracts. Hence, the developed method was encouraging for the quantitative analysis of phenanthrenes in genus .
菲并呋喃类化合物是存在于 属中的生物活性酚类化合物，它们在果皮中的分布多于果肉。对 属果皮中的菲并呋喃类化合物的研究揭示了其具有成为有价值生物材料的潜力。本文建立并验证了一种采用高效液相色谱（HPLC）对生物活性菲并呋喃类化合物进行定量分析的方法。使用先前从 中分离得到的菲并呋喃类化合物（1-3）进行校准曲线绘制，其浓度范围为 0.625-20.00μg/ml，令人满意的决定系数（R）为 0.999。分离出的菲并呋喃类化合物的检测限（LOD）和定量限（LOQ）值范围分别为 0.78-0.89 和 2.38-2.71μg/ml。日内和日间精密度范围分别为 0.25-7.58%。在 1.25、2.5 和 5.0μg/ml 的浓度下，分离出的菲并呋喃类化合物的回收率为 95-100%。此外，在所研究的所有果皮提取物中均发现了菲并呋喃类化合物（1-3）。因此，所建立的方法对于 属中菲并呋喃类化合物的定量分析具有鼓励意义。
