在植物中开发高效的 Prime Editor 2 系统。
Development of a highly efficient prime editor 2 system in plants.
机构信息
Key Laboratory of Rice Genetic Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230031, P.R. China.
College of Agronomy, Anhui Agricultural University, Hefei, 230036, P.R. China.
出版信息
Genome Biol. 2022 Jul 25;23(1):161. doi: 10.1186/s13059-022-02730-x.
Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
低效率严重限制了主导编辑（PE）系统在植物中的应用。在这项研究中，我们通过堆叠各种优化策略，包括更新 PE 结构为 PEmax 以及在组合启动子的控制下表达带有结构基序的工程化 pegRNA，开发了一个增强型植物主导编辑器 2 系统，enpPE2。在 T 水稻植物中，enpPE2 显示出 64.58%至 77.08%的编辑频率，远高于未修饰的 pPE2 的频率。我们的结果表明，enpPE2 系统为植物基因组的精确修饰提供了一个强大而有力的工具。
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