Ocular and Stem Cell Translational Research, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Ocular and Stem Cell Translational Research, National Eye Institute, National Institutes of Health, Bethesda, MD 20892, USA.
STAR Protoc. 2022 Jul 20;3(3):101582. doi: 10.1016/j.xpro.2022.101582. eCollection 2022 Sep 16.
RPE tissues are derived from induced pluripotent stem cells (iPSCs) to model retinal diseases and as a replacement therapy for macular degeneration. Here, we developed a robust and efficient directed differentiation protocol to generate pure RPE cells that form a polarized monolayer. This protocol describes how to set up RPE differentiation and to obtain a pure population that expresses mature RPE markers and forms strong tight junctions. For complete details on the use and execution of this protocol, please refer to Sharma et al., 2019, Sharma et al., 2021 and Miyagishima et al. (2021).
RPE 组织来源于诱导多能干细胞（iPSCs），用于模拟视网膜疾病，并作为黄斑变性的替代疗法。在这里，我们开发了一种强大而高效的定向分化方案，可生成形成极化单层的纯 RPE 细胞。该方案描述了如何建立 RPE 分化并获得表达成熟 RPE 标志物并形成强紧密连接的纯群体。有关此方案的使用和执行的完整详细信息，请参阅 Sharma 等人，2019 年，Sharma 等人，2021 年和 Miyagishima 等人。（2021 年）。
