Wang Tso-Fu, Liou Yu-Shan, Chang Hsin-Hou, Yang Shang-Hsien, Li Chi-Cheng, Wang Jen-Hung, Sun Der-Shan
Department of Hematology and Oncology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 97002, Taiwan.
Department of Medicine, College of Medicine, Tzu Chi University, Hualien 97004, Taiwan.
J Clin Med. 2022 Jul 18;11(14):4169. doi: 10.3390/jcm11144169.
This study investigated the correlation of body mass index (BMI) and proinflammatory cytokine levels with hematopoietic stem cell (HSC) mobilization triggered by granulocyte colony-stimulating factor (G-CSF). Stem cell donors (n = 309) were recruited between August 2015 and January 2018 and grouped into four groups according to their BMI: underweight (BMI < 18.5 kg/m2, n = 10), normal (18.5 kg/m2 ≦ BMI < 25 kg/m2, n = 156), overweight (25 kg/m2 ≦ BMI < 30 kg/m2, n = 102), and obese (BMI ≧ 30 kg/m2, n = 41). The participants were then administered with five doses of G-CSF and categorized as good mobilizers (CD34 ≧ 180/μL, n = 15, 4.85%) and poor mobilizers (CD34 ≦ 25/μL, n = 14, 4.53%) according to the number of CD34+ cells in their peripheral blood after G-CSF administration. The correlation between BMI and HSC mobilization was then analyzed, and the levels of proinflammatory cytokines in the plasma from good and poor mobilizers were examined by ProcartaPlex Immunoassay. Results showed that BMI was highly correlated with G-CSF-triggered HSC mobilization (R2 = 0.056, p < 0.0001). Compared with poor mobilizers, good mobilizers exhibited higher BMI (p < 0.001) and proinflammatory cytokine [interferon gamma (IFN-γ) (p < 0.05), interleukin-22 (IL-22) (p < 0.05), and tumor necrosis factor alpha (TNF-α) levels (p < 0.05)]. This study indicated that BMI and proinflammatory cytokine levels are positively correlated with G-CSF-triggered HSC mobilization.
本研究调查了体重指数（BMI）和促炎细胞因子水平与粒细胞集落刺激因子（G-CSF）触发的造血干细胞（HSC）动员之间的相关性。在2015年8月至2018年1月期间招募了干细胞供者（n = 309），并根据他们的BMI分为四组：体重过轻（BMI < 18.5 kg/m²，n = 10）、正常（18.5 kg/m² ≤ BMI < 25 kg/m²，n = 156）、超重（25 kg/m² ≤ BMI < 30 kg/m²，n = 102）和肥胖（BMI ≥ 30 kg/m²，n = 41）。然后给参与者注射五剂G-CSF，并根据G-CSF给药后外周血中CD34⁺细胞的数量将其分类为良好动员者（CD34 ≥ 180/μL，n = 15，4.85%）和不良动员者（CD34 ≤ 25/μL，n = 14，4.53%）。然后分析BMI与HSC动员之间的相关性，并通过ProcartaPlex免疫测定法检测良好和不良动员者血浆中促炎细胞因子的水平。结果显示BMI与G-CSF触发的HSC动员高度相关（R² = 0.056，p < 0.0001）。与不良动员者相比，良好动员者的BMI更高（p < 0.001），促炎细胞因子[干扰素γ（IFN-γ）（p < 0.05）、白细胞介素-22（IL-22）（p < 0.05）和肿瘤坏死因子α（TNF-α）水平（p < 0.05）]也更高。本研究表明BMI和促炎细胞因子水平与G-CSF触发的HSC动员呈正相关。
