Filardo Simone, Di Pietro Marisa, Sessa Rosa
Department of Public Health and Infectious Diseases, University of Rome "Sapienza", 00185 Rome, Italy.
Life (Basel). 2022 Jul 15;12(7):1065. doi: 10.3390/life12071065.
Currently, still possesses a significant impact on public health, with more than 130 million new cases each year, alongside a high prevalence of asymptomatic infections (approximately 80% in women and 50% in men). infection involves a wide range of different cell types, from cervical epithelial cells, testicular Sertoli cells to Synovial cells, leading to a broad spectrum of pathologies of varying severity both in women and in men. Several two-dimensional in vitro cellular models have been employed for investigating host-cell interaction, although they present several limitations, such as the inability to mimic the complex and dynamically changing structure of in vivo human host-tissues. Here, we present a brief overview of the most cutting-edge three-dimensional cell-culture models that mimic the pathophysiology of in vivo human tissues and organs for better translating experimental findings into a clinical setting. Future perspectives in the field of research are also provided.
目前，（该疾病）仍对公众健康产生重大影响，每年新增病例超过1.3亿，同时无症状感染的患病率很高（女性约为80%，男性约为50%）。（该疾病）感染涉及多种不同类型的细胞，从宫颈上皮细胞、睾丸支持细胞到滑膜细胞，导致女性和男性出现一系列严重程度不同的病理状况。已经采用了几种二维体外细胞模型来研究宿主 - 细胞相互作用，尽管它们存在一些局限性，例如无法模拟体内人类宿主组织复杂且动态变化的结构。在这里，我们简要概述了最前沿的三维细胞培养模型，这些模型模拟体内人类组织和器官的病理生理学，以便更好地将实验结果转化为临床应用。还提供了该研究领域的未来展望。
